| Effects of two volatile anesthetics and a volatile convulsant on the excitatory and inhibitory amino acid responses in dissociated CNS neurons of the rat. | |
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MedLine Citation:
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PMID: 1667416 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Effects of two volatile anesthetics [halothane (Hal) and enflurane (Enf)] and a volatile convulsant [hexafluorodiethyl ether (HFE)] on amino acid-induced membrane currents in neurons dissociated from the nucleus tractus solitarius of the rat were examined. The dissociated neurons were voltage clamped in the whole-cell mode of the patch-clamp technique. All drugs were applied with a microperfusion system, termed the "Y-tube" method. 2. The glutamate (Glu)-induced excitatory response was slightly reduced by both the anesthetics. The responses to three agonists at Glu receptor were depressed by Hal (10(-3) M) in the rank order of quisqualate greater than N-methyl-D-aspartate greater than kainate. HFE slightly increased the Glu response at a high concentration of 2 x 10(-3) M. 3. The gamma-aminobutyric acid (GABA)-induced chloride current (ICl) was enhanced by both anesthetics. The dissociation constant (Kd) for the enhancement was 2.3 x 10(-4) M for Hal and 2.1 x 10(-4) M for Enf, and the Hill coefficient was 1.6 for Hal and 1.5 for Enf. HFE depressed the GABA response with a Kd of 8.7 x 10(-5) M and a Hill coefficient of 0.84. 4. Hal (10(-3) M) and Enf (10(-3) M) decreased the Kd of the GABA concentration-response curve from 3.5 x 10(-6) to 10(-6) and 1.9 x 10(-6) M, respectively, without changing the maximum response or the Hill coefficient (1.5). In the presence of HFE (10(-4) M), the Kd was increased to 1.4 x 10(-5) M and the Hill coefficient was slightly changed to 1.2.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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M Wakamori; Y Ikemoto; N Akaike |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neurophysiology Volume: 66 ISSN: 0022-3077 ISO Abbreviation: J. Neurophysiol. Publication Date: 1991 Dec |
Date Detail:
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Created Date: 1992-06-10 Completed Date: 1992-06-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375404 Medline TA: J Neurophysiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2014-21 Citation Subset: IM |
Affiliation:
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Department of Neurophysiology, Tohoku University School of Medicine, Sendai, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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pharmacology* Animals Brain Stem / physiology* Dose-Response Relationship, Drug Electrophysiology / methods Enflurane / pharmacology* Flurothyl / pharmacology* Glutamates / pharmacology Glycine / pharmacology Halothane / pharmacology* Kainic Acid / pharmacology N-Methylaspartate / pharmacology Neurons / drug effects, physiology* Quisqualic Acid / pharmacology Rats Rats, Inbred Strains Receptors, GABA-A / drug effects, physiology Receptors, Neurotransmitter / drug effects, physiology gamma-Aminobutyric Acid / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Glutamates; 0/Receptors, GABA-A; 0/Receptors, Neurotransmitter; 0/barbiturate receptor; 13838-16-9/Enflurane; 151-67-7/Halothane; 333-36-8/Flurothyl; 487-79-6/Kainic Acid; 52809-07-1/Quisqualic Acid; 56-12-2/gamma-Aminobutyric Acid; 56-40-6/Glycine; 6384-92-5/N-Methylaspartate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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