Document Detail


Effects of tripchlorolide on the epididymides and testes of rats.
MedLine Citation:
PMID:  11250778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To further evaluate the antifertility effects of tripchlorolide, a derivative of triptolide produced at the extraction procedure of Tripterygium wilfordii Hook. f., in male rats and to investigate its sites and possible mechanisms of action. METHODS: In male rats, tripchlorolide was given by oral garage at a dose of 50 micrograms.kg-1.d-1 for 5 weeks, fertility was assessed by mating tests, and biochemical indices and light microscopic observation of the epididymides and testes were also performed. RESULTS: Administration of tripchlorolide at 50 micrograms.kg-1.d-1 for 3 weeks did not influence the fertility of male rats, but 5-week treatment rendered the rats infertile. The density and motility of spermatozoa collected from cauda epididymides were reduced significantly. The epididymal weights, as well as the L-carnitine concentration and alpha-glucosidase content in the epididymal fluid were decreased. There were no significant differences in alpha-glucosidase and acid phosphatase (ACP) in caput epididymal homogenates between the control and the experimental rats. Obvious morphological changes were observed in the epididymal spermatozoa, mainly including head and tail separation or acrosome curving. Sloughed spermatids were found in the seminifeous and epididymal tubules. In testicular homogenates, tripchlorolide had no influence on the lactate dehydrogenase-C4 (LDH-C4) and hyaluronidase activities. No apparent lesions were observed in the seminiferous and epididymal epithelium. CONCLUSION: At the dose level employed, tripchlorolide has a significant effect on the fertility in male rats and the primary sites of action may be spermatids and testicular and epididymal spermatozoa.
Authors:
Z P Wang; Z P Gu; L Cao; Y Xu; G D You; B Y Mao; S Z Qian
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Asian journal of andrology     Volume:  1     ISSN:  1008-682X     ISO Abbreviation:  Asian J. Androl.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  2001-06-08     Completed Date:  2001-06-21     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  100942132     Medline TA:  Asian J Androl     Country:  China    
Other Details:
Languages:  eng     Pagination:  121-5     Citation Subset:  IM    
Affiliation:
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Weight / drug effects
Contraceptive Agents, Male / pharmacology
Diterpenes / pharmacology*
Epididymis / drug effects*
Fertility / drug effects
Male
Organ Size / drug effects
Phenanthrenes*
Rats
Rats, Sprague-Dawley
Sperm Motility / drug effects
Testis / anatomy & histology,  drug effects*
Chemical
Reg. No./Substance:
0/Contraceptive Agents, Male; 0/Diterpenes; 0/Phenanthrenes; 132368-08-2/tripchlorolide

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