| Effects of triethylene tetraamine on telomerase activity and proliferation in HeLa cells. | |
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MedLine Citation:
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PMID: 15109985 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Telomerase, which is required to maintain telomeres, has attracted considerable attention as a target for anticancer therapy. In this study, we investigated the inhibition of HeLa cell telomerase activity and cell cycle progression by triethylene tetraamine (TETA), using a modified telomeric repeat amplification protocol (TRAP) assay, and flow cytometry. TETA inhibited telomerase activity in HeLa cell extracts, with an IC50 of about 7.8 microM. Coupled with this inhibition, TETA also increased the proportion of cells in the G1 phase of the cell cycle in a dose-dependent manner. These findings demonstrate that TETA is a potent inhibitor of telomerase in micromolar concentrations, and inhibits the proliferation of HeLa cells by arresting them in G1. |
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Authors:
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Fei Yin; Jianhui Liu; Xiaojun Peng |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cell biology international Volume: 28 ISSN: 1065-6995 ISO Abbreviation: Cell Biol. Int. Publication Date: 2004 |
Date Detail:
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Created Date: 2004-04-27 Completed Date: 2005-03-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
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Languages: eng Pagination: 287-91 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Fine Chemicals, Dalian University of Technology, 158 Zhong-shan Road, Dalian 116012, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle
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drug effects* Enzyme Activation / drug effects G1 Phase / drug effects* Hela Cells Humans Telomerase / metabolism* Triethylenetetramine / toxicity* |
| Chemical | |
Reg. No./Substance:
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112-24-3/Triethylenetetramine; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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