Document Detail

Effects of transforming growth factor-alpha on chicken adipocyte precursor cells in vitro.
MedLine Citation:
PMID:  1402526     Owner:  NLM     Status:  MEDLINE    
The hyperplastic capacity of adipose tissue resides in a group of fibroblast-like adipocyte precursor cells. There is evidence to suggest that their proliferation and differentiation is regulated by insulin-like growth factor-I (IGF-I) and transforming growth factor-beta (TGF-beta) but there is less information about other growth factors which may also participate in adipocyte precursor cell hyperplasia. Transforming growth factor-alpha (TGF-alpha) is a 50 amino acid polypeptide which has been shown to stimulate proliferation in both neoplastic and normal cell types acting through the epidermal growth factor (EGF) receptor. We have studied the regulation of DNA synthesis and the activity of lipoprotein lipase by TGF-alpha in chicken adipocyte precursor cells in vitro. Both TGF-alpha and EGF stimulated incorporation of [3H]thymidine into DNA in a dose-dependent manner. TGF-alpha was approximately 180-fold more potent than EGF. Addition of TGF-alpha in combination with IGF-I, TGF-beta 1 or platelet-derived growth factor produced a synergistic increase in DNA synthesis. Short-term incubation with TGF-alpha reduced lipoprotein lipase activity by 23%. These results show that TGF-alpha is a potent mitogen in these adipocyte precursor cells and can inhibit their differentiation in vitro and may participate in the regulation of adipose tissue development in vivo.
S C Butterwith; C D Peddie; C Goddard
Related Documents :
16424156 - Cutting edge: deficiency in the e3 ubiquitin ligase cbl-b results in a multifunctional ...
3457016 - Transforming growth factor type beta regulation of actin mrna.
1733936 - Purification and characterization of transforming growth factor-beta 2.3 and -beta 1.2 ...
2273056 - Evidence for c-myc in the signaling pathway for tgf-beta in well-differentiated human c...
17581616 - Baff antagonist attenuates the development of skin fibrosis in tight-skin mice.
7378106 - A highly topically active corticosteroid. preliminary report.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of endocrinology     Volume:  134     ISSN:  0022-0795     ISO Abbreviation:  J. Endocrinol.     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-10-30     Completed Date:  1992-10-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  163-8     Citation Subset:  IM    
Department of Cellular and Molecular Biology, AFRC Institute of Animal Physiology and Genetics Research, Roslin, Midlothian, U.K.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adipose Tissue / drug effects*,  metabolism,  pathology
Cell Differentiation / drug effects
Cell Division / drug effects
Cells, Cultured
DNA / biosynthesis
Dose-Response Relationship, Drug
Epidermal Growth Factor / pharmacology
Lipoprotein Lipase / metabolism
Mitogens / pharmacology*
Transforming Growth Factor alpha / pharmacology*
Reg. No./Substance:
0/Mitogens; 0/Transforming Growth Factor alpha; 62229-50-9/Epidermal Growth Factor; 9007-49-2/DNA; EC Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Mitochondrial breakage induced by the herbicide paraquat in cultured human lung cells.
Next Document:  Effects of the daily administration of gonadotrophin-releasing hormone on the anterior pituitary gla...