Document Detail


Effects of topography on the functional development of human neural progenitor cells.
MedLine Citation:
PMID:  20198656     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have fabricated a topographical substrate with a packed polystyrene bead array for the development of cell-based assay systems targeting voltage-gated calcium channels (VGCCs). Human neural progenitor cells (H945RB.3) cultured on both flat and topographical substrates were analyzed in terms of morphological spreading, neuronal commitment, resting membrane potential (V(m)) establishment and VGCC function development. We found, by SEM imaging, that arrayed substrates, formed with both sub-micrometer (of 0.51 microm in mean diameter) and micrometer (of 1.98 microm in mean diameter) beads, were capable of promoting the spreading of the progenitor cells as compared with the flat polystyrene surfaces. With the micrometer beads, it was found that arrayed substrates facilitated the neural progenitor cells' maintenance of less negative V(m) values upon differentiation with bFGF starvation, which favored predominant neuronal commitment. Almost all the progenitor cells were responsive to 50 mM K(+) depolarization with an increase in [Ca(2+)](i) either before or upon differentiation, suggesting the expression of functional VGCCs. Compared to the flat polystyrene surfaces, microbead arrayed substrates facilitated the development of higher VGCC responsiveness by the progenitor cells upon differentiation. The enhancement of both VGCC responsiveness and cell spreading by arrays of micrometer beads was most significant on day 14 into differentiation, which was the latest time point of measurement in this study. This study thus rationalized the possibility for future substrate topography engineering to manipulate ion channel function and to meet the challenge of low VGCC responsiveness found in early drug discovery.
Authors:
Ze-Zhi Wu; William S Kisaalita; Lina Wang; Angela L Zachman; Yiping Zhao; Kowser Hasneen; Dave Machacek; Steven L Stice
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  106     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-05-26     Completed Date:  2010-08-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  649-59     Citation Subset:  IM    
Affiliation:
Key Laboratory of Biorheological Science and Technology under the State Ministry of Education, 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing, PR China.
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MeSH Terms
Descriptor/Qualifier:
Calcium / metabolism
Calcium Channels / physiology
Cell Culture Techniques
Cell Line
Humans
Microscopy, Electron, Scanning
Microspheres
Neuromuscular Depolarizing Agents / metabolism
Neurons / cytology,  physiology*
Polystyrenes
Potassium / metabolism
Stem Cells / cytology,  physiology*
Chemical
Reg. No./Substance:
0/Calcium Channels; 0/Neuromuscular Depolarizing Agents; 0/Polystyrenes; 7440-09-7/Potassium; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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