Document Detail

Effects to HeLa Cells of the Inhibition of Survivin by Antisense RNA.
MedLine Citation:
PMID:  12040397     Owner:  NLM     Status:  Publisher    
To study the function of survivin in cancer cells, survivin cDNA was amplified from HeLa cells by RT-PCR. Then the coding fragment was subcloned into an inducible eukaryotic expression plasmid pHC in reverse direction.The obtained plasmid pHSC was transfected into HeLa cells by Lipofectamine. An inducible cell line which can express antisense RNA of survivin with 2 mmol/L Zn(2 ) was obtained by the selection with 800 nmol/L G418 solution. The expression of antisense RNA of survivin inhibited the expression of survivin protein. Inhibition of survivin expression suppressed the proliferation of HeLa cells and its cell cycle. And it also sensitized HeLa cells to chemotherapeutics.
Zhu-Zhong Mei; Yan Dong; Zhi-Xian Sun
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Publication Detail:
Journal Detail:
Title:  Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica     Volume:  33     ISSN:  0582-9879     ISO Abbreviation:  Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao     Publication Date:  2001  
Date Detail:
Created Date:  2002-May-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  20730160R     Medline TA:  Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  547-551     Citation Subset:  -    
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
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