| Effects of titanium particle size on osteoblast functions in vitro and in vivo. | |
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MedLine Citation:
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PMID: 15755807 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The formation of titanium (Ti)-wear particles during the lifetime of an implant is believed to be a major component of loosening due to debris-induced changes in bone cell function. Radiographic evidence indicates a loss of fixation at the implant-bone interface, and we believe that the accumulation of Ti particles may act on the bone-remodeling process and impact both long- and short-term implant-fixation strengths. To determine the effects of various sizes of the Ti particles on osteoblast function in vivo, we measured the loss of integration strength around Ti-pin implants inserted into a rat tibia in conjunction with Ti particles from one of four size-groups. Implant integration is mediated primarily by osteoblast adhesion/focal contact pattern, viability, proliferation and differentiation, and osteoclast recruitment at the implant site in vivo. This study demonstrates the significant attenuation of osteoblast function concurrent with increased expression of receptor activator of nuclear factor kappaB ligand (RANKL), a dominant signal for osteoclast recruitment, which is regulated differentially, depending on the size of the Ti particle. Zymography studies have also demonstrated increased activities of matrix metalloproteinases (MMP) 2 and 9 in cells exposed to larger Ti particles. In summary, all particles have adverse effects on osteoblast function, resulting in decreased bone formation and integration, but different mechanisms are elicited by particles of different sizes. |
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Authors:
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Moon G Choi; Hae S Koh; Daniel Kluess; Daniel O'Connor; Anshu Mathur; George A Truskey; Janet Rubin; David X F Zhou; K-L Paul Sung |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. Date: 2005-03-08 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 102 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2005 Mar |
Date Detail:
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Created Date: 2005-03-23 Completed Date: 2005-05-03 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 4578-83 Citation Subset: IM |
Affiliation:
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Department of Orthopedic Surgery, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biomechanics Bone Nails Carrier Proteins / genetics Cell Proliferation / drug effects Cell Survival / drug effects Matrix Metalloproteinase 2 / metabolism Matrix Metalloproteinase 9 / metabolism Membrane Glycoproteins / genetics Osseointegration / drug effects, genetics, physiology Osteoblasts / cytology, drug effects*, physiology* Particle Size Prosthesis Failure RANK Ligand RNA, Messenger / genetics, metabolism Rats Rats, Wistar Tibia / cytology, surgery Titanium / administration & dosage, toxicity* |
| Grant Support | |
ID/Acronym/Agency:
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AR14918/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Membrane Glycoproteins; 0/RANK Ligand; 0/RNA, Messenger; 7440-32-6/Titanium; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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