Document Detail

Effects of tetrodotoxin and ion replacements on the short-circuit current induced by Escherichia coli enterotoxin STa across the colon of the gerbil (Gerbillu cheesmani) in different dietary states.
MedLine Citation:
PMID:  15970465     Owner:  NLM     Status:  MEDLINE    
The effects of mucosally added Escherichia coli heat stable enterotoxin (STa, 30 ng mL(-1)) on the basal short-circuit current (Isc in microA cm(-2)) across stripped and unstripped sheets of proximal, mid and distal colon were investigated. Samples were taken from fed, starved (4 days, water ad lib) and undernourished (50% of control food intake for 21 days) gerbils (Gerbillus cheesmani). The effect of neurotoxin tetrodotoxin (TTX 10 microM) and the effect of replacing chloride by gluconate or the effect of removing bicarbonate from bathing buffer on the maximum increase in Isc induced by E. coli heat stable enterotoxin STa were also investigated. The results showed that there is a segmental difference both in the basal and STa-induced Isc. Also, STa is more effective in the proximal than distal colon in the three feeding conditions. Undernourishment raised the STa-induced Isc in the three regions of the colon. In fed and starved gerbils part of STa-induced Isc in the proximal colon was chloride-dependent, while the other was bicarbonate-dependent; in the mid colon, the STa-induced Isc was bicarbonate-dependent only. In the three regions of the colon taken from undernourished gerbil, the STa-induced Isc was both chloride-and bicarbonate-dependent. The increase in STa-induced Isc as a results of undernourishment in proximal and mid colon was chloride-dependent, while in the distal colon, it was both chloride- and bicarbonate-dependent.
Fawzia Y Al-Balool
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative biochemistry and physiology. Toxicology & pharmacology : CBP     Volume:  141     ISSN:  1532-0456     ISO Abbreviation:  Comp. Biochem. Physiol. C Toxicol. Pharmacol.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-07-12     Completed Date:  2005-09-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100959500     Medline TA:  Comp Biochem Physiol C Toxicol Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-7     Citation Subset:  IM    
Department of Biological Sciences, Faculty of Science, Kuwait University, P.O. Box 5969, Safat 13060, State of Kuwait.
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MeSH Terms
Bacterial Toxins / pharmacology*
Bicarbonates / pharmacology
Chlorides / metabolism,  pharmacology
Colon / drug effects*,  metabolism*
Enterotoxins / pharmacology*
Escherichia coli*
Escherichia coli Proteins / pharmacology
Gluconates / metabolism,  pharmacology
Ions / metabolism,  pharmacology
Malnutrition / physiopathology
Starvation / physiopathology
Tetrodotoxin / pharmacology*
Reg. No./Substance:
0/Bacterial Toxins; 0/Bicarbonates; 0/Buffers; 0/Chlorides; 0/Enterotoxins; 0/Escherichia coli Proteins; 0/Gluconates; 0/Ions; 0/heat stable toxin (E coli); 4368-28-9/Tetrodotoxin; 526-95-4/gluconic acid

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