| Effects of terbium chelate structure on dipicolinate ligation and the detection of Bacillus spores. | |
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MedLine Citation:
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PMID: 22071082 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Terbium-sensitized luminescence and its applicability towards the detection of Bacillus spores such as anthrax are of significant interest to research in biodefense and medical diagnostics. Accordingly, we have measured the effects of terbium chelation upon the parameters associated with dipicolinate ligation and spore detection. Namely, the dissociation constants, intrinsic brightness, luminescent lifetimes, and biological stabilities for several Tb(chelate)(dipicolinate)(x) complexes were determined using linear, cyclic, and aromatic chelators of differing structure and coordination number. This included the chelator array of NTA, BisTris, EGTA, EDTA, BAPTA, DO2A, DTPA, DO3A, and DOTA (respectively, 2,2',2″-nitrilotriacetic acid; 2,2-bis(hydroxymethyl)-2,2',2″-nitrilotriethanol; ethylene glycol-bis(2-aminoethyl ether)-N,N,N',N'-tetraacetic acid; ethylenediamine-N,N,N',N'-tetraacetic acid; 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; 1,4,7,10-tetraazacyclododecane-1,7-diacetic acid; diethylenetriamine-N,N,N',N″,N″-pentaacetic acid; 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid; and 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid). Our study has revealed that the thermodynamic and temporal emission stabilities of the Tb(chelate)(dipicolinate)(x) complexes are directly related to chelate rigidity and a ligand stoichiometry of x=1, and that chelators possessing either aromaticity or low coordination numbers are destabilizing to the complexes when in extracts of an extremotolerant Bacillus spore. Together, our results demonstrate that both Tb(EDTA) and Tb(DO2A) are chemically and biochemically stable and thus applicable as respectively low and high-cost luminescent reporters for spore detection, and thereby of significance to institutions with developing biodefense programs. |
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Authors:
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L S Barnes; K R Kaneshige; J S Strong; K Tan; H F von Bremen; R Mogul |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-14 |
Journal Detail:
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Title: Journal of inorganic biochemistry Volume: 105 ISSN: 1873-3344 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-11-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7905788 Medline TA: J Inorg Biochem Country: - |
Other Details:
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Languages: ENG Pagination: 1580-1588 Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Chemistry Department, California State Polytechnic University, Pomona, 3801 W. Temple Ave., Pomona, CA 91768, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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