Document Detail


Effects of temporary cooling, and of different explantation and storage conditions, on the subsequent development of post-implantation rat embryos in vitro.
MedLine Citation:
PMID:  3238594     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rat embryos explanted at head fold stage were stored under various levels of hypothermia prior to culture. The storage media were Hanks' Balanced Salt Solution (BSS), 50% rat serum with 50% Dulbecco's Modification of Eagle's Medium (standard medium), or 100% rat serum. The media were gassed with 5% O2/5% CO2/90% N2 or 20% O2/5% CO2/75% N2. Subsequent development of embryos after storage at temperatures between 10 degrees C and 30 degrees C for 5 hr in Hanks' BSS, or for 5-10 hr in standard medium or serum, was similar to that of controls. Some embryos developed well even after storage for 48 hr in standard medium. Development was poorer after storage at 0 degrees C or 5 degrees C, and after storage at all temperatures in ungassed Hanks' or standard medium (pH greater than 8.0). Differences in oxygen level had little effect. For routine explantation at room temperature in (ungassed) phosphate-buffered saline solutions such as Hanks', it is recommended that the delay before transferring the embryos to the culture incubator not exceed 2-3 hr.
Authors:
S G Kachilele; D A New
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Teratology     Volume:  38     ISSN:  0040-3709     ISO Abbreviation:  Teratology     Publication Date:  1988 Oct 
Date Detail:
Created Date:  1989-05-01     Completed Date:  1989-05-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0153257     Medline TA:  Teratology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  381-7     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Malawi, Chancellor College, Zomba.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cold Temperature
Culture Media
Embryo, Mammalian / physiology*
Embryonic and Fetal Development*
Freezing
Mice
Mice, Inbred Strains
Organ Culture Techniques
Rats
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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