Document Detail

Effects of temporary cooling, and of different explantation and storage conditions, on the subsequent development of post-implantation rat embryos in vitro.
MedLine Citation:
PMID:  3238594     Owner:  NLM     Status:  MEDLINE    
Rat embryos explanted at head fold stage were stored under various levels of hypothermia prior to culture. The storage media were Hanks' Balanced Salt Solution (BSS), 50% rat serum with 50% Dulbecco's Modification of Eagle's Medium (standard medium), or 100% rat serum. The media were gassed with 5% O2/5% CO2/90% N2 or 20% O2/5% CO2/75% N2. Subsequent development of embryos after storage at temperatures between 10 degrees C and 30 degrees C for 5 hr in Hanks' BSS, or for 5-10 hr in standard medium or serum, was similar to that of controls. Some embryos developed well even after storage for 48 hr in standard medium. Development was poorer after storage at 0 degrees C or 5 degrees C, and after storage at all temperatures in ungassed Hanks' or standard medium (pH greater than 8.0). Differences in oxygen level had little effect. For routine explantation at room temperature in (ungassed) phosphate-buffered saline solutions such as Hanks', it is recommended that the delay before transferring the embryos to the culture incubator not exceed 2-3 hr.
S G Kachilele; D A New
Related Documents :
25213684 - Substrate versatility of polyhydroxyalkanoate producing glycerol grown bacterial enrich...
6118034 - Selective recovery of living microfilariae from onchocerca volvulus nodules. determinat...
1614954 - Comparison of heat-shock-induced and lipopolysaccharide-induced protein changes and tum...
16653054 - Induction of cold stability of microtubules in cultured tobacco cells.
10885204 - Value of superficial cultures in diagnosing neonatal sepsis.
17216444 - Influence of growth medium on cometabolic degradation of polycyclic aromatic hydrocarbo...
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Teratology     Volume:  38     ISSN:  0040-3709     ISO Abbreviation:  Teratology     Publication Date:  1988 Oct 
Date Detail:
Created Date:  1989-05-01     Completed Date:  1989-05-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0153257     Medline TA:  Teratology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  381-7     Citation Subset:  IM    
Department of Biology, University of Malawi, Chancellor College, Zomba.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cold Temperature
Culture Media
Embryo, Mammalian / physiology*
Embryonic and Fetal Development*
Mice, Inbred Strains
Organ Culture Techniques
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Embryonic catch-up growth after exposure to the ketone body D,L,-beta-hydroxybutyrate in vitro.
Next Document:  Teratogenic and postnatal developmental studies of morphine in Sprague-Dawley rats.