Document Detail


Effects of temperature and nutrient availability on plasma membrane lipid composition in Scots pine roots during growth initiation.
MedLine Citation:
PMID:  14757583     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We studied the effects of root zone temperature (RZT) and nutrient availability on free sterols and phospholipids in the plasma membrane (PM) and on PM-ATPase activity in roots of 1-year-old Scots pine (Pinus sylvestris L.) seedlings during growth initiation in the spring. Seedlings were grown for 6 weeks in hydroponic cultures with low (0.5 mM N; LN) or high (3 mM N; HN) nutrient availability. The root zone was subjected to slow warming (SW) and fast warming (FW) treatments while maintaining similar air temperatures in both treatments. Decreases in the amount of phospholipids and in the phospholipid/free sterol ratio, an increase in the degree of saturation of phospholipid fatty acids and changes in free sterol composition were observed during root growth initiation. Changes in lipid composition of the PM associated with the cold deacclimation process were detected at RZTs above 9 degrees C. Nutrient availability affected the lipid composition of the PM only when RZT was increased slowly. When RZT increased from 4 to 6 degrees C in the SW treatment, the degree of saturation of phospholipid fatty acids decreased, especially in HN seedlings. The sitosterol/stigmasterol ratio remained higher in HN seedlings than in LN seedlings. After an RZT of 9 degrees C had been reached in the SW treatment, HN caused increases in the saturation of phospholipid fatty acids and root PM-ATPase activity, and a decrease in the phospholipid/free sterol ratio. Possible effects of changes in PM lipid composition on root growth and PM-ATPase activity are discussed.
Authors:
Sari Iivonen; Pekka Saranpää; Marja-Liisa Sutinen; Elina Vapaavuori
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tree physiology     Volume:  24     ISSN:  0829-318X     ISO Abbreviation:  Tree Physiol.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-02-03     Completed Date:  2005-11-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100955338     Medline TA:  Tree Physiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  437-46     Citation Subset:  IM    
Affiliation:
Finnish Forest Research Institute, Suonenjoki Research Station, Juntintie 40, 77600 Suonenjoki, Finland.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / metabolism
Cell Membrane / chemistry
Membrane Lipids / analysis
Phospholipids / analysis
Phytosterols / analysis
Pinus sylvestris / chemistry,  growth & development*
Plant Roots / chemistry,  growth & development*
Temperature
Trees / chemistry,  growth & development*
Chemical
Reg. No./Substance:
0/Membrane Lipids; 0/Phospholipids; 0/Phytosterols; EC 3.6.1.-/Adenosine Triphosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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