| Effects of "systemic" budesonide concentrations on in vitro allergen-induced activation of blood mononuclear cells isolated from asthmatic patients. | |
MedLine Citation:
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PMID: 7573828 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Blood levels of inhaled corticosteroids are significantly lower than those measured in the lung, but their concentration could still have anti-inflammatory effects. To determine whether budesonide, at concentrations similar to those obtained in blood after drug inhalation (10(-9) M), could downregulate the allergen-induced activation of mononuclear cells, we studied 21 atopic patients, sensitized to Dermatophagoides pteronyssinus (Der p). On blood mononuclear cells, isolated from these patients, incubated with Der p allergen extract and with or without budesonide, we evaluated: 1) the proliferative response of T cells; 2) the expression of two surface activation markers, the HLA-DR antigens and the interleukin (IL)-2 receptors; and 3) the release of cytokines known to modulate the allergic processes. Allergen-induced T-cell proliferation was associated with increased HLA-DR antigen and IL-2 receptor expression (P < 0.001), and with increased release of IL-2, interferon-gamma (IFN-gamma), IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), and granulocyte/macrophage colony-stimulating factor (GM-CSF). The addition of budesonide at the beginning of the cell cultures induced a dose-dependent inhibition of T-cell proliferation, still significant (P < 0.05) at the lowest concentrations tested (10(-9) and 10(-10) M). A significant inhibitory effect on T-cell proliferation was also present when budesonide (10(-9) M) was added to the cell cultures 3 or 5 days after the beginning of the cell cultures.(ABSTRACT TRUNCATED AT 250 WORDS) |
Authors:
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S Oddera; M Silvestri; O Sacco; S Lantero; M C Morelli; G A Rossi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Allergy Volume: 50 ISSN: 0105-4538 ISO Abbreviation: Allergy Publication Date: 1995 May |
Date Detail:
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Created Date: 1995-11-02 Completed Date: 1995-11-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7804028 Medline TA: Allergy Country: DENMARK |
Other Details:
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Languages: eng Pagination: 397-404 Citation Subset: IM |
Affiliation:
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Division of Pneumology, G. Gaslini Institute, Genoa, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Allergens / immunology* Antigens, Dermatophagoides Asthma / blood*, immunology* Budesonide Cell Separation Cytokines / metabolism Female Glucocorticoids / administration & dosage*, therapeutic use Glycoproteins / immunology HLA-DR Antigens / immunology Humans Lymphocyte Activation* / drug effects Male Monocytes / immunology*, metabolism Osmolar Concentration Pregnenediones / administration & dosage*, therapeutic use Receptors, Interleukin-2 / immunology T-Lymphocytes / immunology |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/Antigens, Dermatophagoides; 0/Cytokines; 0/Glucocorticoids; 0/Glycoproteins; 0/HLA-DR Antigens; 0/Pregnenediones; 0/Receptors, Interleukin-2; 51333-22-3/Budesonide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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