Document Detail


Effects of synthetic cyclic AMP analogs on amylase exocytosis from rat pancreatic acini.
MedLine Citation:
PMID:  7533648     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Diastereomers of adenosine 3',5'-phosphorothioate activate cAMP-dependent protein kinases (cAMP-PK) in vitro. We found that these compounds are highly selective tools to monitor cAMP-dependent PKA activation and its effect on amylase exocytosis from pancreatic acini. In permeabilized rat acinar cells, (Sp)-cAMPS dose-dependently stimulated amylase secretion, while (Rp)-cAMPS inhibited (Sp)-cAMPS-induced amylase release. In intact rat acini, 8-Br-(Rp)-cAMPS reduced the secretory responses to secretin, vasoactive intestinal polypeptide (VIP), 8-Br-cAMP, and 8-Br-(Sp)-cAMPS, but not to cerulein. Another derivative, dibutyryl-(Rp)-cAMPS, induced a small inhibitory effect against 8-Br-(Sp)-cAMPS and VIP, which was overlapped by an unspecific stimulatory effect on amylase exocytosis induced by the degradation product butyrate. Furthermore, (Sp)-5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole-3',5'- monophosphorothioate ((Sp)-5,6-DCl-cBIMPS), a specific cAMP-PK activator, induced a maximal induction of cAMP-PK activity, but its stimulation of amylase secretion was less than that by secretin. (Sp)-5,6-DCl-cBIMPS regulated the phosphorylation of several proteins, which were also affected by secretin. However, secretin had additional effects. Its action was most likely mediated by a dual effect on the cAMP and the calcium pathway. Our results indicate that the cAMP-dependent pathway is involved in amylase exocytosis from rat pancreatic acini.
Authors:
C Schäfer; H Steffen; H Printz; B Göke
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of physiology and pharmacology     Volume:  72     ISSN:  0008-4212     ISO Abbreviation:  Can. J. Physiol. Pharmacol.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1995-04-12     Completed Date:  1995-04-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372712     Medline TA:  Can J Physiol Pharmacol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  1138-47     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Philipps-University of Marburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amylases / secretion*
Animals
Autoradiography
Calcium / pharmacology
Cells, Cultured
Cyclic AMP / analogs & derivatives,  pharmacology*
Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors,  metabolism*
Dose-Response Relationship, Drug
Exocytosis / drug effects*
Female
Pancreas / drug effects*,  secretion
Phosphoproteins / metabolism
Rats
Rats, Wistar
Secretin / metabolism
Stereoisomerism
Chemical
Reg. No./Substance:
0/Phosphoproteins; 1393-25-5/Secretin; 60-92-4/Cyclic AMP; 7440-70-2/Calcium; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases; EC 3.2.1.-/Amylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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