Document Detail


Effects of surfactant subfractions on preterm rabbit lung function.
MedLine Citation:
PMID:  2356104     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Surfactant isolated from adult rabbits was separated into large aggregate forms (LA) and small aggregate forms (SA) by centrifugation at 40,000 x g. LA contain SP-A and the surfactant lipophilic proteins SP-B and SP-C and are surface-active in vitro, whereas SA lack the surfactant proteins and have poor surface properties. Premature rabbits were treated with LA or SA and ventilated to evaluate the effects of LA and SA in vivo. There were dose-response effects of LA at doses between 10 and 50 mg total lipid/kg: dynamic compliances increased from 0.4 to 0.8 mL/cm H2O/kg and pressure-volume curves improved significantly, whereas SA did not improve any of the measurements at any dose. Organic solvent extracts of SA made up into LA also did not improve in vivo function. When 1.5% SP-B and SP-C isolated from calf surfactant was added to organic solvent extracts of SA, the surfactant had good in vitro surface properties and increased maximum lung volumes. However, this surfactant did not improve dynamic compliances or lung stability on pressure-volume curves. Because the lipids from SA seemed to limit the compliance response, we tried to identify differences in lipids from LA and SA. Phospholipids from SA reconstituted with SP-BC did not function well in vivo, whereas phospholipids from LA with SP-BC were similar to LA surfactant. The neutral lipid fractions from LA or SA did not contribute very much to or inhibit function. Fatty acid composition of the phospholipids and phosphatidylcholine from LA and SA were not remarkably different.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
T Yamada; M Ikegami; A H Jobe
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Pediatric research     Volume:  27     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-07-20     Completed Date:  1990-07-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  592-8     Citation Subset:  IM    
Affiliation:
Harbor-UCLA Medical Center, Department of Pediatrics, Torrance 90509.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Fatty Acids / analysis
Lung / drug effects*,  physiology
Phospholipids / analysis
Pulmonary Surfactants / isolation & purification,  pharmacology*
Rabbits
Solvents
Surface Tension
Grant Support
ID/Acronym/Agency:
HD-11932/HD/NICHD NIH HHS; HD-12714/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Phospholipids; 0/Pulmonary Surfactants; 0/Solvents

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