Document Detail

Effects of supplementation with free glutamine and the dipeptide alanyl-glutamine on parameters of muscle damage and inflammation in rats submitted to prolonged exercise.
MedLine Citation:
PMID:  19885855     Owner:  NLM     Status:  MEDLINE    
In this study, we investigated the effect of the supplementation with the dipeptide L-alanyl-L-glutamine (DIP) and a solution containing L-glutamine and L-alanine on plasma levels markers of muscle damage and levels of pro-inflammatory cytokines and glutamine metabolism in rats submitted to prolonged exercise. Rats were submitted to sessions of swim training for 6 weeks. Twenty-one days prior to euthanasia, the animals were supplemented with DIP (n = 8) (1.5, a solution of free L-glutamine (1 and free L-alanine (0.61 (G&A, n = 8) or water (control (CON), n = 8). Animals were killed at rest before (R), after prolonged exercise (PE-2 h of exercise). Plasma concentrations of glutamine, glutamate, tumour necrosis factor-alpha (TNF-alpha), prostaglandin E2 (PGE2) and activity of creatine kinase (CK), lactate dehydrogenase (LDH) and muscle concentrations of glutamine and glutamate were measured. The concentrations of plasma TNF-alpha, PGE2 and the activity of CK were lower in the G&A-R and DIP-R groups, compared to the CON-R. Glutamine in plasma (p < 0.04) and soleus muscle (p < 0.001) was higher in the DIP-R and G&A-R groups relative to the CON-R group. G&A-PE and DIP-PE groups exhibited lower concentrations of plasma PGE2 (p < 0.05) and TNF-alpha (p < 0.05), and higher concentrations of glutamine and glutamate in soleus (p < 0.001) and gastrocnemius muscles (p < 0.05) relative to the CON-PE group. We concluded that supplementation with free L-glutamine and the dipeptide LL-alanyl-LL-glutamine represents an effective source of glutamine, which may attenuate inflammation biomarkers after periods of training and plasma levels of CK and the inflammatory response induced by prolonged exercise.
Vinicius Fernandes Cruzat; Marcelo Macedo Rogero; Julio Tirapegui
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  28     ISSN:  1099-0844     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-28     Completed Date:  2010-03-19     Revised Date:  2013-05-27    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  England    
Other Details:
Languages:  eng     Pagination:  24-30     Citation Subset:  IM    
Copyright Information:
2009 John Wiley & Sons, Ltd.
Department of Food Science and Experimental Nutrition, University of São Paulo, Brazil.
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MeSH Terms
Alanine / pharmacology
Creatine Kinase / blood,  metabolism
Dietary Supplements
Dinoprostone / blood
Dipeptides / pharmacology*
Glutamic Acid / blood
Glutamine / blood,  pharmacology*
Inflammation Mediators / metabolism*
L-Lactate Dehydrogenase / blood,  metabolism
Muscle, Skeletal / metabolism*
Physical Conditioning, Animal*
Rats, Wistar
Tumor Necrosis Factor-alpha / blood
Reg. No./Substance:
0/Dipeptides; 0/Inflammation Mediators; 0/Tumor Necrosis Factor-alpha; 363-24-6/Dinoprostone; 56-41-7/Alanine; 56-85-9/Glutamine; 56-86-0/Glutamic Acid; EC Dehydrogenase; EC Kinase; U5JDO2770Z/alanylglutamine

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