Document Detail


Effects of supplementation with free glutamine and the dipeptide alanyl-glutamine on parameters of muscle damage and inflammation in rats submitted to prolonged exercise.
MedLine Citation:
PMID:  19885855     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we investigated the effect of the supplementation with the dipeptide L-alanyl-L-glutamine (DIP) and a solution containing L-glutamine and L-alanine on plasma levels markers of muscle damage and levels of pro-inflammatory cytokines and glutamine metabolism in rats submitted to prolonged exercise. Rats were submitted to sessions of swim training for 6 weeks. Twenty-one days prior to euthanasia, the animals were supplemented with DIP (n = 8) (1.5 g.kg(-1)), a solution of free L-glutamine (1 g.kg(-1)) and free L-alanine (0.61 g.kg(-1)) (G&A, n = 8) or water (control (CON), n = 8). Animals were killed at rest before (R), after prolonged exercise (PE-2 h of exercise). Plasma concentrations of glutamine, glutamate, tumour necrosis factor-alpha (TNF-alpha), prostaglandin E2 (PGE2) and activity of creatine kinase (CK), lactate dehydrogenase (LDH) and muscle concentrations of glutamine and glutamate were measured. The concentrations of plasma TNF-alpha, PGE2 and the activity of CK were lower in the G&A-R and DIP-R groups, compared to the CON-R. Glutamine in plasma (p < 0.04) and soleus muscle (p < 0.001) was higher in the DIP-R and G&A-R groups relative to the CON-R group. G&A-PE and DIP-PE groups exhibited lower concentrations of plasma PGE2 (p < 0.05) and TNF-alpha (p < 0.05), and higher concentrations of glutamine and glutamate in soleus (p < 0.001) and gastrocnemius muscles (p < 0.05) relative to the CON-PE group. We concluded that supplementation with free L-glutamine and the dipeptide LL-alanyl-LL-glutamine represents an effective source of glutamine, which may attenuate inflammation biomarkers after periods of training and plasma levels of CK and the inflammatory response induced by prolonged exercise.
Authors:
Vinicius Fernandes Cruzat; Marcelo Macedo Rogero; Julio Tirapegui
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  28     ISSN:  1099-0844     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-28     Completed Date:  2010-03-19     Revised Date:  2013-05-27    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  England    
Other Details:
Languages:  eng     Pagination:  24-30     Citation Subset:  IM    
Copyright Information:
2009 John Wiley & Sons, Ltd.
Affiliation:
Department of Food Science and Experimental Nutrition, University of São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Alanine / pharmacology
Animals
Creatine Kinase / blood,  metabolism
Dietary Supplements
Dinoprostone / blood
Dipeptides / pharmacology*
Glutamic Acid / blood
Glutamine / blood,  pharmacology*
Inflammation Mediators / metabolism*
L-Lactate Dehydrogenase / blood,  metabolism
Muscle, Skeletal / metabolism*
Physical Conditioning, Animal*
Rats
Rats, Wistar
Tumor Necrosis Factor-alpha / blood
Chemical
Reg. No./Substance:
0/Dipeptides; 0/Inflammation Mediators; 0/Tumor Necrosis Factor-alpha; 363-24-6/Dinoprostone; 56-41-7/Alanine; 56-85-9/Glutamine; 56-86-0/Glutamic Acid; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.7.3.2/Creatine Kinase; U5JDO2770Z/alanylglutamine

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