| Effects of subunit cross-linking on the properties of crotoxin. | |
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MedLine Citation:
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PMID: 2402766 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Crotoxin was cross-linked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride. Cross-linked crotoxin had the expected amino-terminal amino acids, amino acid composition, behavior on SDS-PAGE and an 80% reduction of reactable lysine residues. It was also non-toxic, had reduced immunological cross-reactivity toward both poly- and monoclonal antibodies raised to the basic subunit of crotoxin and had lost greater than 95% of its phospholipase activity. Loss of toxicity is due to either subunit cross-linking or the modification of essential residues. |
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Authors:
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B W Lennon; D Plummer; I I Kaiser |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Toxicon : official journal of the International Society on Toxinology Volume: 28 ISSN: 0041-0101 ISO Abbreviation: Toxicon Publication Date: 1990 |
Date Detail:
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Created Date: 1990-10-26 Completed Date: 1990-10-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1307333 Medline TA: Toxicon Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 718-22 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, University of Wyoming, Laramie 82071. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Cross-Linking Reagents / pharmacology Crotalid Venoms / toxicity* Crotoxin / analysis, immunology, toxicity* Hydroxylamine Hydroxylamines / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Cross-Linking Reagents; 0/Crotalid Venoms; 0/Hydroxylamines; 7803-49-8/Hydroxylamine; 9007-40-3/Crotoxin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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