| Effects of a stroma-free hemoglobin and perfluorochemical combination on ultrastructure and function of the isolated rat kidney. | |
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MedLine Citation:
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PMID: 8771240 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Experimental perfusions of isolated rat kidneys were performed with flow rates adjusted to produce comparable glomerular filtration rates (GFR) in control and experimental groups. The experimental perfusate, consisting of Krebs-Ringer bicarbonate (KRB) containing 3.5% (uncrosslinked) stroma-free hemoglobin (SFH) plus 3.5% of the perfluorochemical (PFC) Fluosol-DA, was found to produce only 48% as much urine as the control perfusate consisting of KRB containing 7% dextran (without either SFH or PFC). Perfusion with the experimental SFH/PFC mixture was associated with mean fractional reabsorptions of sodium 3.3% greater and of potassium 5.1% lesser than perfusion with the control KRB (with dextran) solution (p < .05). The SFH/PFC mixture was localized histochemically to the capillaries and urinary spaces of glomeruli; and to the apical microvilli, basal laminae, and intracytoplasmic vacuoles of proximal renal tubular cells. Since the glomerular filtration rate was not a factor, decremental urine production appears to be associated with increased reabsorption of sodium, excretion of potassium, and proximal tubular uptake of the experimental SFH/PFC mixture by endocytosis. |
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Authors:
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R M Millis; J D Barber; W A Anderson; O Dehkordi |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Renal failure Volume: 17 ISSN: 0886-022X ISO Abbreviation: Ren Fail Publication Date: 1995 Nov |
Date Detail:
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Created Date: 1996-10-01 Completed Date: 1996-10-01 Revised Date: 2008-05-21 |
Medline Journal Info:
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Nlm Unique ID: 8701128 Medline TA: Ren Fail Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 675-87 Citation Subset: IM |
Affiliation:
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Department of Physiology, College of Medicine, Howard University Washington, DC 20059, USA. rocket@CLDC.howard.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Fluorocarbons / administration & dosage, pharmacology* Glomerular Filtration Rate / drug effects* Hemoglobins / administration & dosage, pharmacology* Isotonic Solutions / administration & dosage, pharmacology Kidney / drug effects*, metabolism, ultrastructure Male Perfusion / methods Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Fluorocarbons; 0/Hemoglobins; 0/Isotonic Solutions; 0/hemoglobin, stroma free |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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