Document Detail

Effects of streptozotocin- and alloxan-induced diabetes mellitus on mouse follicular and early embryo development.
MedLine Citation:
PMID:  2526873     Owner:  NLM     Status:  MEDLINE    
Mice were made diabetic by intraperitoneal injection of streptozotocin or alloxan. Germinal vesicle breakdown in the ovarian follicles at 8 h after hCG in control animals (57%) was significantly greater than in streptozotocin-(24%) and alloxan-(42%) diabetic animals (P less than 0.001). This delay in oocyte maturation was reversible by in-vivo insulin administration to diabetic mice. A developmental delay was also found for embryos recovered from diabetic mice. This developmental delay extended into the 72 h in-vitro cultures. Compared to control embryos, those from alloxan- and streptozotocin-treated mice demonstrated marked impairment in development as assessed by (1) distribution of developmental cell stages at each observation period and (2) rates of development which increasingly diverged at each observation period. In diabetic mice treated with insulin in vivo, the percentage of 2-cell embryos recovered increased. Furthermore, in streptozotocin- and alloxan-animals treated with insulin, the rate of in-vitro development of embryos, as well as their developmental stage distribution improved. We therefore suggest that uncontrolled diabetes mellitus, as well as contributing to the development of congenital malformations, may deleteriously affect reproductive performance both before fertilization and at the very earliest gestational stages.
M P Diamond; K H Moley; A Pellicer; W K Vaughn; A H DeCherney
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  86     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-08-31     Completed Date:  1989-08-31     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1-10     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT 06510.
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MeSH Terms
Alloxan / pharmacology
Diabetes Mellitus, Experimental / drug therapy,  embryology,  physiopathology*
Embryonic and Fetal Development*
Insulin / therapeutic use
Mice, Inbred Strains
Oocytes / physiology*
Ovarian Follicle / physiology*
Pregnancy in Diabetics / drug therapy,  embryology,  physiopathology*
Streptozocin / pharmacology
Zygote / drug effects
Reg. No./Substance:
11061-68-0/Insulin; 18883-66-4/Streptozocin; 50-71-5/Alloxan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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