Document Detail

Effects of storage time and temperature on urinary enzymes.
MedLine Citation:
PMID:  1678320     Owner:  NLM     Status:  MEDLINE    
We measured three renal tubular brush-border enzymes (lactate dehydrogenase, LDH, EC; gamma-glutamyltransferase, GGT, EC; and alkaline phosphatase, AP, EC in morning urine samples from 48 healthy subjects to check whether different storage times and temperatures could modify enzyme concentrations. Short-term (24 h) storage time at room temperature or 4 degree C does not affect urinary enzyme activity. A few days of freezing, at -20 or -70 degrees C, dramatically lowers LDH and AP values; GGT is partially preserved only at -70 degrees C, if the sample has been previously centrifuged. Urinary enzymes investigated in this study are extremely labile at low temperatures.
E Matteucci; G Gregori; L Pellegrini; R Navalesi; O Giampietro
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical chemistry     Volume:  37     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1991 Aug 
Date Detail:
Created Date:  1991-09-19     Completed Date:  1991-09-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1436-41     Citation Subset:  IM    
Cattedra di Malattie del Metabolismo, Istituto di Clinica Medica II, Università degli Studi di Pisa, Italy.
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MeSH Terms
Alkaline Phosphatase / urine*
Kidney Tubules / enzymology*
L-Lactate Dehydrogenase / urine*
Microvilli / enzymology*
Preservation, Biological*
Reference Values
Spectrophotometry, Ultraviolet
Time Factors
gamma-Glutamyltransferase / urine*
Reg. No./Substance:
EC Dehydrogenase; EC; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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