Document Detail


Effects of storage time and temperature on urinary enzymes.
MedLine Citation:
PMID:  1678320     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We measured three renal tubular brush-border enzymes (lactate dehydrogenase, LDH, EC 1.1.1.27; gamma-glutamyltransferase, GGT, EC 2.3.2.2; and alkaline phosphatase, AP, EC 3.1.3.1) in morning urine samples from 48 healthy subjects to check whether different storage times and temperatures could modify enzyme concentrations. Short-term (24 h) storage time at room temperature or 4 degree C does not affect urinary enzyme activity. A few days of freezing, at -20 or -70 degrees C, dramatically lowers LDH and AP values; GGT is partially preserved only at -70 degrees C, if the sample has been previously centrifuged. Urinary enzymes investigated in this study are extremely labile at low temperatures.
Authors:
E Matteucci; G Gregori; L Pellegrini; R Navalesi; O Giampietro
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical chemistry     Volume:  37     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1991 Aug 
Date Detail:
Created Date:  1991-09-19     Completed Date:  1991-09-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1436-41     Citation Subset:  IM    
Affiliation:
Cattedra di Malattie del Metabolismo, Istituto di Clinica Medica II, Università degli Studi di Pisa, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / urine*
Humans
Kidney Tubules / enzymology*
Kinetics
L-Lactate Dehydrogenase / urine*
Microvilli / enzymology*
Preservation, Biological*
Reference Values
Spectrophotometry, Ultraviolet
Temperature
Time Factors
gamma-Glutamyltransferase / urine*
Chemical
Reg. No./Substance:
EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.3.2.2/gamma-Glutamyltransferase; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Identification of deletion mutations and three new genes at the familial polyposis locus.
Next Document:  Despite expression in embryonic visceral mesoderm, H2.0 is not essential for Drosophila visceral mus...