Document Detail

Effects of storage on serum ionized calcium and pH from horses with normal and abnormal ionized calcium concentrations.
MedLine Citation:
PMID:  12660955     Owner:  NLM     Status:  Publisher    
It has been previously shown that Ca(I) concentration is stable in serum collected from healthy horses for 10 days if stored at 40 degrees C. This may not be true for horses with abnormal Ca(I) concentrations. Thus the stability of ionized calcium (Ca(I)) concentration and pH measurement in serum from horses with both normal and abnormal Ca(I) concentrations stored for various times at 40 degrees C and -10 degrees C was evaluated. Our results indicated that serum Ca(I) concentration was stable throughout 7 days of cold or frozen storage, after being received by the Clinical Chemistry Laboratory. Serum Ca(I) concentration showed a significant decrease by 14 days of frozen storage (-10 degrees C). Serum pH showed a statistically significant increase by 7 days of cold storage, and within 3 days of frozen storage. If equine serum is collected, handled and stored anaerobically, and kept cold or frozen, Ca(I) concentration can be accurately measured for approximately 7 days after collection, regardless of the health status of the animal. An accurate measurement of pH may be made within 3 days of cold or 1 day of frozen storage.
Patricia A. Schenck; Dennis J. Chew; Charles L. Brooks
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Publication Detail:
Journal Detail:
Title:  Veterinary clinical pathology / American Society for Veterinary Clinical Pathology     Volume:  25     ISSN:  0275-6382     ISO Abbreviation:  Vet Clin Pathol     Publication Date:  1996  
Date Detail:
Created Date:  2003-Mar-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9880575     Medline TA:  Vet Clin Pathol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  118-120     Citation Subset:  -    
Buckeye Feed Mills, Inc., P.O. Box 505, 330 East Schultz Avenue, Dalton, OH 44618.
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