Document Detail


Effects of sphincterotomy and pudendal nerve transection on the anal sphincter in a rat model.
MedLine Citation:
PMID:  19571711     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Our objective was to define anal resting pressure and electromyography of the normal rat anal sphincter and investigate the short-term effects of both mechanical trauma to the anal sphincter muscles and pudendal nerve transection. METHODS: Forty-five virgin female Sprague-Dawley rats were randomly allotted to three groups: controls (n = 21), sphincterotomy (n = 12), and pudendal nerve transection (n = 12). Anal pressure was monitored using a saline-filled balloon connected to a pressure transducer. Anal pressure and electromyography of the anal sphincter with use of a needle electrode were recorded both before and after injury or succinylcholine administration. RESULTS: Anal pressure data were consistent with rhythmic pressure contractions. Succinylcholine significantly reduced both pressure and electromyography signals. Electromyography amplitude and frequency decreased after nerve transection but not after sphincterotomy. The histology showed that the rat anal anatomy has muscular components that compare with human anatomy. The sphincterotomy group showed injury to the anal sphincters and the sphincter anatomy of the nerve transection group appeared similar to the control group. The anal pressure wave appears to be created by synergistic activity of both striated and smooth muscle of the anal sphincter. CONCLUSION: The female rat is a suitable and reliable model for studying effect of direct and indirect injury to the anal sphincters.
Authors:
Massarat Zutshi; Levilester B Salcedo; Paul J Zaszczurynski; Tracy L Hull; Robert S Butler; Margot S Damaser
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diseases of the colon and rectum     Volume:  52     ISSN:  1530-0358     ISO Abbreviation:  Dis. Colon Rectum     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-02     Completed Date:  2009-07-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372764     Medline TA:  Dis Colon Rectum     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1321-9     Citation Subset:  IM    
Affiliation:
Department of Colorectal Surgery, Cleveland Clinic, Cleveland, Ohio, USA. zutshim@ccf.org
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MeSH Terms
Descriptor/Qualifier:
Action Potentials / physiology
Anal Canal / injuries*,  innervation,  surgery*
Animals
Disease Models, Animal*
Electromyography
Female
Muscle Contraction / physiology
Muscle Strength
Muscle, Striated / innervation*,  physiopathology*,  surgery
Pressure
Rats
Rats, Sprague-Dawley
Reproducibility of Results

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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