| Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets. | |
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MedLine Citation:
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PMID: 22272087 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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This study was developed to provide further information on the intestinal barrier permeability and the tight junction protein expression in weaned piglets fed with different levels of soybean agglutinin (SBA). Twenty-five weaned crossbred barrows (Duroc × Landrace × Yorkshire) were selected and randomly allotted to five groups, each group with five replicates. The piglets in the control group were not fed with leguminous products. 0.05, 0.1, 0.15 and 0.2% SBA was added to the control diet to form four experimental diets, respectively. After the experimental period of 7 days (for each group), all the piglets were anesthetized with excess procaine and slaughtered. The d-lactic acid in plasma and the Ileal mucosa diamine oxidase (DAO) was analyzed to observe the change in the intestinal permeability. The tight junction proteins occludin and ZO-1 in the jejunum tissue distribution and relative expression were detected by immunohistochemistry and Western Blot. The results illustrated that a high dose of SBA (0.1-0.2%) could increase the intestinal permeability and reduce piglet intestinal epithelial tight junction protein occludin or ZO-1 expression, while low dose of SBA (0.05% of total diet) had no significant affects. The contents of DAO, d-lactic acid, occludin or ZO-1, had a linear relationship with the SBA levels (0-0.2%) in diets. The high dose SBA (0.1-0.2%) could increase the intestinal permeability and reduce piglet intestinal epithelial tight junction protein occludin or ZO-1 expression, while low dose of SBA (0.05% of total diet) had no affects. |
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Authors:
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Yuan Zhao; Guixin Qin; Zewei Sun; Dongsheng Che; Nan Bao; Xiaodong Zhang |
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Publication Detail:
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Type: Journal Article Date: 2011-11-29 |
Journal Detail:
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Title: International journal of molecular sciences Volume: 12 ISSN: 1422-0067 ISO Abbreviation: Int J Mol Sci Publication Date: 2011 |
Date Detail:
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Created Date: 2012-01-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101092791 Medline TA: Int J Mol Sci Country: Switzerland |
Other Details:
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Languages: eng Pagination: 8502-12 Citation Subset: - |
Affiliation:
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Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun 130118, China; E-Mails: zhaoyuan4CL52@126.com (Y.Z.); chedongsheng@163.com (D.C.); baonan203@163.com (N.B.). |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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