| Effects of some metal ions on rainbow trout erythrocytes glutathione S-transferase enzyme: an in vitro study. | |
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MedLine Citation:
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PMID: 23057421 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Glutathione S-transferase enzyme (GST) (EC 2.5.1.18) was purified from rainbow trout erythrocytes, and some characteristics of the enzyme and effects of some metal ions on enzyme activity were investigated. For this purpose, erythrocyte glutathione S-transferase enzyme which has 16.54 EU/mg protein specific activities was purified 11,026-fold by glutathione-agarose affinity chromatography with a yield of 59%. Temperature was kept under control (+4°C) during purification. Enzyme purification was checked by performing SDS-PAGE. Optimal pH, stable pH, optimal temperature, and K(M) and V(max) values for GSH and 1-chloro-2, 4-dinitrobenzene (CDNB) were also determined for the enzyme. In addition, IC(50) values, K(i) constants and the type of inhibition were determined by means of Line-Weaver-Burk graphs obtained for such inhibitors as Ag(+); Cd(2+), Cr(2+) and Mg(2+). |
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Authors:
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Veysel Comakli; Mehmet Ciftci; O Irfan Kufrevioglu |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-12 |
Journal Detail:
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Title: Journal of enzyme inhibition and medicinal chemistry Volume: - ISSN: 1475-6374 ISO Abbreviation: J Enzyme Inhib Med Chem Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101150203 Medline TA: J Enzyme Inhib Med Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Agri Ibrahim Cecen University , Health Services Vocational School TR-04100, Agri , Turkey. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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