| Effects of the soluble fibre pectin on intestinal cell proliferation, fecal short chain fatty acid production and microbial population. | |
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MedLine Citation:
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PMID: 12743440 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: Although pectin, a dietary fibre, has been suggested to possess some trophic effects on the intestine, the mechanisms involved remain unclear. This study aimed to evaluate the effects of pectin on rat intestinal cell proliferation and the intraluminal environment. METHODS: Control and pectin-fed rats were given a fibre-free elemental diet (ED) and an ED containing 2.5% pectin, respectively. On the 15th day, the length, weight and number of Ki-67-positive cells from each intestinal segment, and the short chain fatty acids (SCFAs) and microbial population in the caecum were measured. Plasma glucagon-like peptide-2 (GLP-2) concentration and GLP-2 receptor (GLP-2R) mRNA levels in the epithelium were also determined. RESULTS: Pectin supplementation resulted in significant increases in the length, weight, and number of Ki-67-positive cells in the ileum, caecum and colon. Although pectin supplementation did not affect the caecal microbial flora that produced SCFAs, the caecal SCFA content was significantly increased. Pectin supplementation also induced an increase in the plasma GLP-2 concentration, but did not affect the GLP-2R mRNA levels in the small intestine. CONCLUSIONS: The increases in the caecal SCFAs and plasma GLP-2 levels induced by pectin supplementation may cause mucosal proliferation in the lower intestinal tract. |
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Authors:
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Tetsuya Fukunaga; Masaya Sasaki; Yoshio Araki; Toshihiko Okamoto; Takashi Yasuoka; Tomoyuki Tsujikawa; Yoshihide Fujiyama; Tadao Bamba |
Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Digestion Volume: 67 ISSN: 0012-2823 ISO Abbreviation: Digestion Publication Date: 2003 |
Date Detail:
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Created Date: 2003-05-13 Completed Date: 2003-08-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0150472 Medline TA: Digestion Country: Switzerland |
Other Details:
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Languages: eng Pagination: 42-9 Citation Subset: IM |
Copyright Information:
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Copyright 2003 S. Karger AG, Basel |
Affiliation:
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Division of Gastroenterology, Department of Internal Medicine, Shiga University of Medical Science, Shiga, Japan. tetsufuk@belle.shiga-med.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division / drug effects Colony Count, Microbial Dietary Fiber / administration & dosage* Fatty Acids, Volatile / biosynthesis* Feces / chemistry, microbiology Glucagon-Like Peptide 2 Glucagon-Like Peptides Immunohistochemistry / methods Intestinal Mucosa / cytology*, microbiology Intestine, Large / metabolism*, microbiology Intestine, Small / metabolism*, microbiology Ki-67 Antigen / metabolism Male Pectins / administration & dosage* Peptides / blood RNA, Messenger / metabolism Rats Rats, Wistar Receptors, Glucagon / genetics Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Volatile; 0/Glucagon-Like Peptide 2; 0/Ki-67 Antigen; 0/Pectins; 0/Peptides; 0/RNA, Messenger; 0/Receptors, Glucagon; 0/glucagon-like peptide receptor; 62340-29-8/Glucagon-Like Peptides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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