Document Detail

Effects of sodium phenylbutyrate on differentiation and induction of the P21WAF1/CIP1 anti-oncogene in human liver carcinoma cell lines.
MedLine Citation:
PMID:  16246228     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: To explore the effects of sodium phenylbutyrate on the proliferation, differentiation, cell cycle arrest and induction of the P(21WAF1/CIP1) anti-oncogene in human liver carcinoma cell lines Bel-7402 and HepG2. METHODS: Bel-7402 and HepG2 human liver carcinoma cells were treated with sodium phenylbutyrate at different concentrations. Light microscopy was used to observe morphological changes in the carcinoma cells. Effects on the cell cycle were detected by using flow cytometry. P(21WAF1/CIP1) expression was determined by both reverse transcription-polymerase chain reaction and western blotting. Statistical analysis was performed by using one-way anova and Student's t-test. RESULTS: Sodium phenylbutyrate treatment caused time- and dose-dependent growth inhibition of Bel-7402 and HepG2 cells. This treatment also caused a decline in the proportion of S-phase cells and an increase in the proportion of G(0)/G(1) cells. Sodium phenylbutyrate increased the expression of P(21WAF1/CIP1). CONCLUSIONS: Sodium phenylbutyrate inhibits the proliferation of human liver carcinoma cells Bel-7402 and HepG2, induces partial differentiation, and increases the expression of P(21WAF1/CIP1).
Mei Meng; Jun Mei Jiang; Hui Liu; Cheng Yong In; Ju Ren Zhu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chinese journal of digestive diseases     Volume:  6     ISSN:  1443-9611     ISO Abbreviation:  Chin J Dig Dis     Publication Date:  2005  
Date Detail:
Created Date:  2005-10-25     Completed Date:  2006-01-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088612     Medline TA:  Chin J Dig Dis     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  189-92     Citation Subset:  IM    
Department of Gastroenterology, Shandong Provincial Hospital, Jinan, China.
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MeSH Terms
Carcinoma, Hepatocellular / genetics
Cell Differentiation / drug effects
Cell Line, Tumor
Cyclin-Dependent Kinase Inhibitor p21 / genetics,  metabolism*
Dose-Response Relationship, Drug
Gene Expression Regulation, Neoplastic / drug effects*
Genes, Tumor Suppressor / drug effects*
Interphase / drug effects
Liver Neoplasms / genetics,  metabolism*
Phenylbutyrates / pharmacology*
RNA, Messenger
Reg. No./Substance:
0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Phenylbutyrates; 0/RNA, Messenger

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