Document Detail


Effects of sodium butyrate on growth, differentiation, and apoptosis in head and neck squamous carcinoma cell lines.
MedLine Citation:
PMID:  10748448     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Biologic agents that reverse early changes in the aerodigestive tract mucosa have potential treatment applications for patients with field cancerization of the upper aerodigestive tract. Sodium butyrate (BA) is a normal dietary constituent that induces differentiation and inhibits growth in several malignant cell types in vitro, but its effect on head and neck squamous cell carcinoma (HNSCC) has not been evaluated. METHODS: Using five HNSCC cell lines, the effects of BA on cell proliferation and apoptosis were examined by colorimetric and fluorescence-labeling methods, and the expression of differentiation markers and apoptosis-related proteins were analyzed using Western and Northern blotting, flow cytometry, and cell cycle analysis. RESULTS: BA-induced growth inhibition and apoptosis in HNSCC cells at millimolar concentrations. Apoptosis induction did not depend on the p53 status of the cell lines or on expression of members of the Bcl-2/Bax family. CONCLUSIONS: These results demonstrate that butyrate has activity against HNSCC in vitro and may have clinical applications for management of HNSCC patients.
Authors:
A Gillenwater; C P Zou; M Zhong; R Lotan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Head & neck     Volume:  22     ISSN:  1043-3074     ISO Abbreviation:  Head Neck     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-05-16     Completed Date:  2000-05-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8902541     Medline TA:  Head Neck     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  247-56     Citation Subset:  IM    
Affiliation:
Departments of Head and Neck Surgery, Box 069, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd, Box 069, Houston, TX 77030, USA. agillenwater@notes.mdacc.tmc.edu
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Blotting, Northern
Blotting, Western
Butyrates / pharmacology*
Carcinoma, Squamous Cell / drug therapy,  pathology*
Cell Differentiation / drug effects*
Cell Division / drug effects
Chi-Square Distribution
Flow Cytometry
G1 Phase / drug effects
Genes, bcl-2
Head and Neck Neoplasms / drug therapy,  pathology*
Humans
Reference Values
Tumor Cells, Cultured / drug effects
Tumor Suppressor Protein p53 / analysis
Grant Support
ID/Acronym/Agency:
P50DE11906-02/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Butyrates; 0/Tumor Suppressor Protein p53

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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