Document Detail

Effects of short term triiodothyronine administration to broiler chickens fed methimazole.
MedLine Citation:
PMID:  19272462     Owner:  NLM     Status:  MEDLINE    
The purposes of these experiments were to determine possible relationships among certain indices of lipid metabolism and specific gene expression in chickens (Gallus gallus) fed methimazole (MMI) and the subsequent effects of providing supplemental T3 to relieve the effects of MMI. Male, broiler chickens growing from 14 to 28 days of age were fed diets containing 18% crude protein and either 0 or 1 g MMI/kg of diet. At 28 days, birds received 18% crude protein diets containing either 0 or 1 mg triiodothyronine (T3)/kg. Birds were sampled at 0, 1, 2 & 4 days post relief from MMI or at 0, 3, 6, 9, 24 & 48 h. Measurements taken in the first experiment included in vitro lipogenesis (IVL), malic enzyme (ME), isocitrate dehydrogenase (ICDNADP), aspartate aminotransferase (AST) enzyme activities and the expression of the genes for ME, fatty acid synthase (FAS) and acetyl coenzyme carboxylase (ACC), ICD and AST. The same enzyme activities and gene expressions were assayed over the intervals mentioned above. In vitro lipogenesis was eliminated due to constraints imposed by sampling times. Gene expression was estimated with real time RT-PCR assays. Dietary MMI decreased IVL and ME at 28 days of age. T3 supplementation for 1 day restored both IVL and ME. Continuing T3 replenishment decreased IVL without affecting ME activity. Although MMI decreased ME gene expression, there was only a transitory relationship between enzyme activity and gene expression when apparent thyroid function was restored with exogenous T3. Metabolic changes in response to feeding T3 occurred within a short period, suggesting that changes in intermediary metabolism preceded morphological changes. Furthermore, the thyroid state of the animal will determine responses to exogenous T3.
R W Rosebrough; B A Russell; M P Richards
Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2009-03-06
Journal Detail:
Title:  Comparative biochemistry and physiology. Toxicology & pharmacology : CBP     Volume:  150     ISSN:  1532-0456     ISO Abbreviation:  Comp. Biochem. Physiol. C Toxicol. Pharmacol.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-05-12     Completed Date:  2010-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100959500     Medline TA:  Comp Biochem Physiol C Toxicol Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  72-8     Citation Subset:  IM    
United States Department of Agriculture-Agricultural Research Service, Beltsville Agricultural Research Center, Animal Biosciences and Biotechnology Laboratory, Animal and Natural Resources Institute, Beltsville, MD 20705, USA. bob.rosebrough@ARS.USDA.GOV
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MeSH Terms
Chickens / metabolism*
Energy Metabolism / drug effects,  physiology
Methimazole / administration & dosage*,  toxicity
Thyroid Hormones / metabolism
Triiodothyronine / administration & dosage*,  toxicity
Reg. No./Substance:
0/Thyroid Hormones; 60-56-0/Methimazole; 6893-02-3/Triiodothyronine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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