Document Detail


Effects of sequential exposure to lipopolysaccharide and heat stress on dental pulp cells.
MedLine Citation:
PMID:  16676349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the present study, we examined the effects of sequential exposure to bacterial lipopolysaccharide (LPS) and heat stress on dental pulp cells. LPS induced the proliferation of pulp cells through the activation of p38 MAPK. HSP27 was expressed in cells with or without LPS during the entire period of heat stress, while transiently phosphorylated by short-term heat stress. In LPS-treated cells, short-term heat stress also induced the phosphorylation of HSF1. The immediate phosphorylation of HSF1 and HSP27 in LPS-treated cells by short-term heat stress occurred dependent on the activation of p38 MAPK. However, with long-term heat stress, the activation of HSF1 and induction of HSP27 occurred independent of p38 MAPK. Further, full activation of Akt in LPS-treated cells was immediately induced by short-term heat stress and lasted during the entire period of heat stress. IkappaB alpha was induced and phosphorylated throughout sequential exposure to LPS and heat stress. These results suggest that LPS has the unique effects on the cytoprotection and the cell death of pulp cells during heat stress through the modification and the activation of heat stress responsive molecules, HSF1 and HSP27, and cell survival molecules, Akt and NF-kappaB/IkappaB alpha.
Authors:
Chiaki Kitamura; Tatsuji Nishihara; Yoshiko Ueno; Ker-Kong Chen; Takahiko Morotomi; Junya Yano; Masato Nagayoshi; Masamichi Terashita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  99     ISSN:  0730-2312     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-02     Completed Date:  2007-06-11     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  797-806     Citation Subset:  IM    
Copyright Information:
2006 Wiley-Liss, Inc.
Affiliation:
Division of Pulp Biology, Operative Dentistry, and Endodontics, Department of Cariology and Periodontology, Science of Oral Functions, Kyushu Dental College, 2-6-1 Manazuru, Kokurakita, Kitakyushu 803-8580, Japan. chi-aki-k@kyu-dent.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Proliferation*
DNA-Binding Proteins / metabolism
Dental Pulp* / cytology,  drug effects
Enzyme Activation
HSP27 Heat-Shock Proteins
Heat-Shock Proteins / metabolism
Hot Temperature*
I-kappa B Proteins / metabolism
Lipopolysaccharides / pharmacology*
MAP Kinase Signaling System / physiology
NF-kappa B / metabolism
Neoplasm Proteins / metabolism
Phosphorylation
Proto-Oncogene Proteins c-akt / metabolism
Rats
Transcription Factors / metabolism
p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors,  metabolism
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/HSP27 Heat-Shock Proteins; 0/Heat-Shock Proteins; 0/Hspb1 protein, rat; 0/I-kappa B Proteins; 0/Lipopolysaccharides; 0/NF-kappa B; 0/Neoplasm Proteins; 0/Transcription Factors; 0/heat shock transcription factor; 139874-52-5/NF-kappaB inhibitor alpha; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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