Document Detail

Effects of selected anti-tumor-promoting chemicals on metabolic cooperation between Chinese hamster V79 cells.
MedLine Citation:
PMID:  7516098     Owner:  NLM     Status:  MEDLINE    
Many tumor-promoting chemicals inhibit gap junctional communication between cells. We investigated the possibility that antipromoting chemicals may act inversely and enhance gap junctional communication. The V79/metabolic cooperation assay is an in vitro test that measures gap junctional communication indirectly by determining the extent of metabolic cooperation between mutant and wild-type V79 Chinese hamster lung fibroblasts in culture. Six in vivo antipromoters (caffeine, 3-isobutyl-1-methylxanthine (IBMX), phenidone, dibromoacetophenone, tosylphenylalanine chloromethyl ketone (TPCK), and acetic acid) were tested in this assay to assess their effects on metabolic cooperation. Caffeine, IBMX, phenidone, and dibromoacetophenone had no effect on metabolic cooperation, while TPCK slightly inhibited metabolic cooperation in one V79 assay. Acetic acid appeared to facilitate metabolic cooperation. In tests where an antipromoter was combined with the established tumor promoter phorbol 12-myristate 13-acetate (PMA), acetic acid, caffeine, and IBMX counteracted PMA-induced inhibition of metabolic cooperation, while phenidone, dibromoacetophenone, and TPCK had little effect. These results indicate that some antipromoters interfere with the ability of a tumor-promoting chemical to inhibit metabolic cooperation and suggest that alteration of gap junctional communication can be a mechanism of antipromoter action.
L J Mills; S M Nelson; A R Malcolm
Related Documents :
2731378 - Metabolism of pyrazinamide and allopurinol in hereditary xanthine oxidase deficiency.
7450778 - Further evidence for heterogeneity of glucose-6-phosphate dehydrogenase deficiency in p...
24412238 - (1)h nmr spectroscopic analysis detects metabolic disturbances in rat urine on acute ex...
24661078 - Prokaryotic taxonomic and metabolic diversity of an intermediate salinity hypersaline h...
25163108 - How the timing of weather events influences early development in a large mammal.
23000428 - N-acylation of phosphatidylethanolamine and its biological functions in mammals.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Toxicology and applied pharmacology     Volume:  126     ISSN:  0041-008X     ISO Abbreviation:  Toxicol. Appl. Pharmacol.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-07-12     Completed Date:  1994-07-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0416575     Medline TA:  Toxicol Appl Pharmacol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  338-44     Citation Subset:  IM    
Science Applications International Corporation, U.S. Environmental Protection Agency, Narragansett, Rhode Island 02882.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
1-Methyl-3-isobutylxanthine / pharmacology
Acetic Acid
Acetic Acids / pharmacology
Acetophenones / pharmacology
Anticarcinogenic Agents / pharmacology*
Arachidonic Acid / metabolism
Caffeine / pharmacology
Cell Communication / drug effects*
Cells, Cultured
Gap Junctions / drug effects*
Lipoxygenase Inhibitors / pharmacology
Mutation / drug effects
Phosphodiesterase Inhibitors / pharmacology
Phospholipases A / antagonists & inhibitors
Protease Inhibitors / pharmacology
Pyrazoles / pharmacology
Tosylphenylalanyl Chloromethyl Ketone / pharmacology
Reg. No./Substance:
0/Acetic Acids; 0/Acetophenones; 0/Anticarcinogenic Agents; 0/Lipoxygenase Inhibitors; 0/Phosphodiesterase Inhibitors; 0/Protease Inhibitors; 0/Pyrazoles; 28822-58-4/1-Methyl-3-isobutylxanthine; 402-71-1/Tosylphenylalanyl Chloromethyl Ketone; 506-32-1/Arachidonic Acid; 58-08-2/Caffeine; 64-19-7/Acetic Acid; 92-43-3/phenidone; 99-73-0/4-bromophenacyl bromide; EC 3.1.1.-/Phospholipases A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Dexamethasone, beta-estradiol, and 2,3,7,8-tetrachlorodibenzo-p-dioxin elicit thymic atrophy through...
Next Document:  NADPH-diaphorase positive cells in the chick and rat thymus.