| Effects of saturated, mono-, and polyunsaturated fatty acids on the secretion of apo B containing lipoproteins by Caco-2 cells. | |
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MedLine Citation:
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PMID: 8678919 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We studied the effects of addition of physiological concentrations (0.5 mM) of fatty acids i.e., palmitic (16:0), stearic (18:0), oleic (18:1) and linoleic acid (18:2) on lipoprotein secretion by polarized Caco-2 cells. With saturated fatty acids, secreted lipoproteins were at IDL/LDL density, 1.009 < d < 1.068 g/ml. The numbers of secreted lipoproteins, expressed as secreted apolipoprotein (apo) B, were comparable to control without fatty acid (palmitic acid, 551 +/- 185; stearic acid, 629 +/- 304 and control, 504 +/- 176 ng apo B/4.7 cm2 filter). With unsaturated fatty acids, apo B containing lipoproteins were secreted at chylomicron/VLDL density (d < 1.006 g/ml). Oleic acid caused a two-fold higher secretion of apo B than control (1058 +/- 87 vs. 504 +/- 176 ng/4.7 cm2 filter, P < 0.001). The increase in apo B secretion was attributed to a specific increase in apo B-48. Unsaturated fatty acid caused a two-fold higher secretion of triglyceride than saturated fatty acids but incorporation of newly synthesized lipid into the secreted lipoproteins, measured by incorporation of a fatty acid marker, was 10- to 20-fold higher, indicating preferential translocation of unsaturated triglycerides into lipoproteins. Mixtures rich in either polyunsaturated, monounsaturated, or saturated fatty acids, resembling nutritional fat and oils, were capable of a two-fold stimulation of secretion of apo B containing triglyceride-rich lipoproteins. The triglyceride/apo B ratio in the basolateral medium was higher with the monounsaturated 'olive oil' mixture (12 250 +/- 2000 mol/mol) than with the polyunsaturated 'corn oil' mixture (7830 +/- 2480 mol/mol) and incorporation of newly synthesized lipid into the secreted lipoproteins was 1.5-fold higher as well. In conclusion, unsaturated fatty acids were most potent in stimulating the secretion of apo B by specifically increasing apo B-48 secretion. Unsaturated triglycerides, that contain mainly oleic acid, were more efficiently incorporated into lipoproteins than saturated triglycerides, suggesting preferential translocation by microsomal triglyceride transfer protein. |
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Authors:
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M M van Greevenbroek; G van Meer; D W Erkelens; T W de Bruin |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Atherosclerosis Volume: 121 ISSN: 0021-9150 ISO Abbreviation: Atherosclerosis Publication Date: 1996 Mar |
Date Detail:
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Created Date: 1996-08-15 Completed Date: 1996-08-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0242543 Medline TA: Atherosclerosis Country: IRELAND |
Other Details:
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Languages: eng Pagination: 139-50 Citation Subset: IM |
Affiliation:
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Department of Medicine, Academic Hospital, Utrecht University, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apolipoprotein B-100 Apolipoprotein B-48 Apolipoproteins B / secretion* Butter / analysis Caco-2 Cells / drug effects, secretion Corn Oil / chemistry Culture Media, Conditioned / chemistry Fatty Acids / chemistry, classification, pharmacology* Humans Linoleic Acid Linoleic Acids / pharmacology Lipoproteins / secretion* Oleic Acid Oleic Acids / pharmacology Palmitic Acid Palmitic Acids / pharmacology Plant Oils / chemistry Stearic Acids / pharmacology Triglycerides / secretion |
| Chemical | |
Reg. No./Substance:
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0/Apolipoprotein B-100; 0/Apolipoprotein B-48; 0/Apolipoproteins B; 0/Culture Media, Conditioned; 0/Fatty Acids; 0/Linoleic Acids; 0/Lipoproteins; 0/Oleic Acids; 0/Palmitic Acids; 0/Plant Oils; 0/Stearic Acids; 0/Triglycerides; 112-80-1/Oleic Acid; 2197-37-7/Linoleic Acid; 57-10-3/Palmitic Acid; 57-11-4/stearic acid; 8001-25-0/olive oil; 8001-30-7/Corn Oil; 8029-34-3/Butter |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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