| Effects of saponins from the root bark of Aralia elata on the transport of chondroitin sulfate in Caco-2 cell monolayers and rats. | |
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MedLine Citation:
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PMID: 15930742 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We investigated the intestinal absorption enhancing effect of the saponins from the root bark of Aralia elata (SRBAE) in Caco-2 cell monolayers and rats. SRBAE at concentrations of 0.04% and 0.08% (w/v) decreased the transepithelial electrical resistance (TEER) values and increased the paracellular uptake of chondroitin sulfates (CSs) having different molecular weights (MW 500, 4500, and 18000) in a dose-dependent manner. We also evaluated the cytotoxicity of SRBAE to determine its proper concentration as an absorption enhancer. MTT assay and trypan blue exclusion test indicated that the cytotoxicity of SRBAE at concentrations of 0.04% and 0.08% was negligible. CS (MW 18000) was orally administered with or without SRBAE to rats. The oral administration of SRBAE (250 mg/kg) in 1 h increased the intestinal absorption of CS, by 4.9-fold versus the control (CS alone). Histological examination of the gastrointestinal tissues showed that SRBAE did not cause any damage to tissues. In conclusion, our results suggest that SRBAE acts as an efficient absorption enhancer and makes it easier for hydrophilic molecules to penetrate the intestinal epithelium. |
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Authors:
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Joon-Soo Sim; Hai Lin Zhao; Da Wei Li; So Yean Cho; Choon Sik Jeong; Eun Bang Lee; Yeong Shik Kim |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biological & pharmaceutical bulletin Volume: 28 ISSN: 0918-6158 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-06-02 Completed Date: 2006-01-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
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Languages: eng Pagination: 1043-8 Citation Subset: IM |
Affiliation:
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Natural Products Research Institute, College of Pharmacy, Seoul National University, Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aralia* Biological Transport / drug effects, physiology Caco-2 Cells Chondroitin Sulfates / metabolism* Humans Intestinal Absorption / drug effects*, physiology Male Plant Bark* Plant Roots* Rats Rats, Sprague-Dawley Saponins / isolation & purification, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Saponins; 9007-28-7/Chondroitin Sulfates |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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