Document Detail


Effects of sample preparation conditions on biomolecular solid-state NMR lineshapes.
MedLine Citation:
PMID:  9835049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sample preparation conditions with the 46 kDa enzyme complex of 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, shikimate-3-phosphate (S3P) and glyphosate (GLP) have been examined in an attempt to reduce linewidths in solid-state NMR spectra. The linewidths of 31P resonances associated with enzyme bound S3P and GLP in the lyophilized ternary complex have been reduced to 150 +/- 12 Hz and 125 +/- 7 Hz respectively, by a variety of methods involving additives and freezing techniques.
Authors:
D L Jakeman; D J Mitchell; W A Shuttleworth; J N Evans
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of biomolecular NMR     Volume:  12     ISSN:  0925-2738     ISO Abbreviation:  J. Biomol. NMR     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1999-01-19     Completed Date:  1999-01-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9110829     Medline TA:  J Biomol NMR     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  417-21     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Biophysics, Washington State University, Pullman 99163-4660, USA.
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MeSH Terms
Descriptor/Qualifier:
3-Phosphoshikimate 1-Carboxyvinyltransferase
Alkyl and Aryl Transferases / chemistry*
Bacterial Proteins / chemistry
Freezing
Glycine / analogs & derivatives*,  chemistry
Macromolecular Substances
Magnetic Resonance Spectroscopy / methods*
Polyethylene Glycols
Shikimic Acid / analogs & derivatives*,  chemistry
Specimen Handling*
Trehalose
Grant Support
ID/Acronym/Agency:
GM43215/GM/NIGMS NIH HHS; RR0631401/RR/NCRR NIH HHS; RR12948/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Macromolecular Substances; 0/Polyethylene Glycols; 1071-83-6/glyphosate; 138-59-0/Shikimic Acid; 56-40-6/Glycine; 63959-45-5/shikimic acid-3-phosphate; 99-20-7/Trehalose; EC 2.5.-/Alkyl and Aryl Transferases; EC 2.5.1.19/3-Phosphoshikimate 1-Carboxyvinyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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