| Effects of salvianolic acid B on proliferation, neurite outgrowth and differentiation of neural stem cells derived from the cerebral cortex of embryonic mice. | |
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MedLine Citation:
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PMID: 20602267 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Salvianolic acid B is isolated from Salvia miltiorrhiza, the root of which is widely used as a traditional Chinese medicine to treat stroke. However, little is known about how salvianolic acid B influences growth characteristics of neural stem cells (NSCs). The purpose of the present study was to evaluate the effects of salvianolic acid B on proliferation, neurite outgrowth and differentiation of NSCs derived from the cerebral cortex of embryonic mice using MTT, flow cytometry, immunofluorescence and RT-PCR. It was found that 20 microg mL(-1) and 40 microg mL(-1) salvianolic acid B had similar effects on proliferation of NSCs, and a suitable concentration of salvianolic acid B increased the number of NSCs and their derivative neurospheres. The growth-promoting activity of salvianolic acid B was dependent on and associated with an accumulation in the G2/S-phase cell population. Salvianolic acid B also promoted the neurite outgrowth of NSCs and their differentiation into neurons. The mRNA for tau, GFAP and nestin were present in differentiating neurospheres induced by salvianolic acid B. However, high-level expression of tau mRNA and low-level expression of GFAP mRNA was detected in differentiated cells, in contrast to the control conditions. This collective evidence indicates that exogenous salvianolic acid B is capable of promoting proliferation of neurospheres and differentiation towards the neuronal lineage in vitro and may act in the proliferation of NSCs and may promote NSC differentiation into neuronal cells. |
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Authors:
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GuoQing Guo; Bin Li; YuanYuan Wang; AiJing Shan; WeiZai Shen; Lin Yuan; ShiZhen Zhong |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-03 |
Journal Detail:
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Title: Science China. Life sciences Volume: 53 ISSN: 1869-1889 ISO Abbreviation: Sci China Life Sci Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-07-05 Completed Date: 2010-10-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101529880 Medline TA: Sci China Life Sci Country: China |
Other Details:
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Languages: eng Pagination: 653-62 Citation Subset: IM |
Affiliation:
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Department of Anatomy, Medical College of Jinan University, Guangzhou, 510630, China. tgqguo@jnu.edu.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Benzofurans / pharmacology* Cell Cycle / drug effects Cell Differentiation / drug effects* Cell Proliferation / drug effects* Cerebral Cortex / cytology, embryology*, metabolism Female Flow Cytometry Fluorescent Antibody Technique Glial Fibrillary Acidic Protein / genetics, metabolism Mice Neurites / drug effects* Pregnancy RNA, Messenger / genetics Reverse Transcriptase Polymerase Chain Reaction Stem Cells / cytology, drug effects*, metabolism tau Proteins / genetics |
| Chemical | |
Reg. No./Substance:
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0/Benzofurans; 0/Glial Fibrillary Acidic Protein; 0/RNA, Messenger; 0/tau Proteins; 115939-25-8/salvianolic acid B |
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