| Effects of route and co-administration of recombinant raccoon poxviruses on immune responses and protection against highly pathogenic avian influenza in mice. | |
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MedLine Citation:
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PMID: 22921740 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We previously demonstrated that recombinant raccoonpox (RCN) virus could serve as a vector for an influenza vaccine. RCN constructs expressing the hemagglutinin (HA) from H5N1 viruses were immunogenic in chickens. In the current study, we generated several recombinant RCN constructs expressing influenza (H5N1) antigens and a molecular adjuvant (Heat-Labile enterotoxin B from E. coli: RCN-LTB), demonstrated their expression in vitro, and evaluated their ability to protect mice against H5N1 virus challenge. RCN-HA provided strong protection when administered intradermally (ID), but not intranasally (IN). Conversely, the RCN-neuraminidase (NA) construct was highly efficacious by the IN route and elicited high titers of neutralizing antibodies in mice. Vaccination by combined ID (RCN-HA) and IN (RCN-NA) routes offered mice the best protection against an IN challenge with heterologous H5N1 virus. However, protection was reduced when the different RCN constructs were pre-mixed, perhaps due to reduced expression of antigen. |
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Authors:
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Brock Kingstad-Bakke; Joseph N Brewoo; Le Quynh Mai; Yoshihiro Kawaoka; Jorge E Osorio |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-22 |
Journal Detail:
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Title: Vaccine Volume: - ISSN: 1873-2518 ISO Abbreviation: Vaccine Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406899 Medline TA: Vaccine Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Ltd. |
Affiliation:
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Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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