| Effects of rhizopodin and latrunculin B on the morphology and on the actin cytoskeleton of mammalian cells. | |
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MedLine Citation:
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PMID: 9931358 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of the novel myxobacterial compound rhizopodin on mammalian cells were studied and compared with those of latrunculin B. Both substances induced adherently growing L929 mouse fibroblasts and PtK2 potoroo kidney cells to produce long, narrow, branched extensions or runners. Rhizopodin was more efficient than latrunculin B in that respect (minimal inhibitory concentration with L929 cells 5 nM vs 50 nM), and, in contrast to latrunculin B, its effects were permanent. Rhizopodin-treated cells became much larger than normal cells and were multinucleate, yet stayed alive and biochemically active for several weeks. Latrunculin B-treated cells returned to a quasi-normal state within 3-4 days. But latrunculin B acted faster, with the first effects becoming visible almost immediately upon the addition of the drug, while the first rhizopodin effects were seen 10 min later. Both substances caused reorganization of the actin cytoskeleton. When 100 nM rhizopodin was added to PtK2 cells, the stress fibers began to decay after just 10 min and had disappeared completely after about 3 h. Later there was a gradual restitution of F-actin. Long F-actin fibers were seen within the runners, and only there; in fact, these fibers may be responsible for the development and extension of the runners. The microtubuli network adjusted itself to the new cell morphology, but was not directly impaired by the compound. |
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Authors:
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T M Gronewold; F Sasse; H Lünsdorf; H Reichenbach |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cell and tissue research Volume: 295 ISSN: 0302-766X ISO Abbreviation: Cell Tissue Res. Publication Date: 1999 Jan |
Date Detail:
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Created Date: 1999-02-23 Completed Date: 1999-02-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0417625 Medline TA: Cell Tissue Res Country: GERMANY |
Other Details:
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Languages: eng Pagination: 121-9 Citation Subset: IM |
Affiliation:
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Department of Biology of Natural Products, Gesellschaft für Biotechnologische Forschung, Mascheroder Weg 1, D-38124 Braunschweig, Germany. kra@gbf-braunschweig.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism* Animals Antineoplastic Agents / pharmacology* Bicyclo Compounds, Heterocyclic / pharmacology* Cell Line Cytoskeleton / drug effects, metabolism, ultrastructure* Fibroblasts / metabolism, ultrastructure Fluorescent Antibody Technique Kidney / metabolism, ultrastructure Mice Microscopy, Electron Oxazoles / pharmacology* Thiazoles / pharmacology* Thiazolidines |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Antineoplastic Agents; 0/Bicyclo Compounds, Heterocyclic; 0/Oxazoles; 0/Thiazoles; 0/Thiazolidines; 150346-23-9/rhizopodin; 76343-94-7/latrunculin B |
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