| Effects of retinoic acid combined with interferon-gamma on the expression of major-histocompatibility-complex molecules and intercellular adhesion molecule-1 in human cervical cancer. | |
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MedLine Citation:
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PMID: 9462716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Retinoids and interferons are important regulators of human epithelial cell differentiation and have been successfully used in the clinical treatment of HPV-involved cervical cancer. In this study, 2 HPV-positive human cervical-carcinoma cell lines were analyzed for their surface expression of MHC-Class-I, MHC-Class-II and ICAM-1 surface antigens before and after exposure to all-trans retinoic acid, interferon-gamma and the combination of the 2 compounds. In addition, the effects on HLA-Class-I-mRNA expression were evaluated after such treatments. Both cell lines expressed MHC-Class-I molecules, and their levels were markedly up-regulated after exposure to IFN-gamma. Similarly, MHC-Class-II and ICAM-1 antigens were either induced or significantly up-regulated by IFN-gamma. Exposure to all-trans retinoic acid was also able to significantly increase the expression of MHC-Class-I and ICAM-I antigens as compared with untreated tumor cells. However, unlike IFN-gamma, retinoids were not able to induce the expression of HLA-Class-II surface antigens. Exposure to the combination of IFN-gamma plus retinoic acid significantly up-regulated (in an additive manner) HLA-Class-I and ICAM-1 molecules as compared with the levels obtainable after exposure to IFN-gamma alone. Finally, Northern-blot analysis of HLA-Class-I-mRNA expression confirmed that the activity of both of these biologic response modifiers was at transcriptional level. These data indicate that the combination of these modalities could induce an additive effect on the expression of immunologically important surface antigens on human cervical-cancer cells. These findings, together with the known anti-proliferative effects mediated by retinoids and IFN-gamma on tumor cells, further support the combination of these agents in the treatment of pre-invasive and invasive human cervical cancer. |
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Authors:
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A D Santin; P Hermonat; A Ravaggi; M Chiriva-Internati; J C Hiserodt; E Tian; C A Carter; S Pecorelli; G P Parham |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of cancer. Journal international du cancer Volume: 75 ISSN: 0020-7136 ISO Abbreviation: Int. J. Cancer Publication Date: 1998 Jan |
Date Detail:
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Created Date: 1998-02-19 Completed Date: 1998-02-19 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 254-8 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, University of Arkansas, Little Rock 72205-7199, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Northern Female Histocompatibility Antigens Class I / analysis Humans Intercellular Adhesion Molecule-1 / analysis* Interferon-gamma / administration & dosage* Tretinoin / administration & dosage* Tumor Cells, Cultured Uterine Cervical Neoplasms / chemistry, drug therapy* |
| Chemical | |
Reg. No./Substance:
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0/Histocompatibility Antigens Class I; 126547-89-5/Intercellular Adhesion Molecule-1; 302-79-4/Tretinoin; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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