Document Detail

Effects of retinoic acid on the activation and fusion of mouse alveolar macrophages induced by 1 alpha,25-dihydroxyvitamin D3.
MedLine Citation:
PMID:  3503549     Owner:  NLM     Status:  MEDLINE    
1 alpha,25-dihydroxyvitamin D3 [1 alpha,25(OH)2D3] directly induces both fusion and cytotoxicity in murine alveolar macrophages. Unlike 1 alpha,25(OH)2D3, retinoic acid per se did not induce fusion of alveolar macrophages, but it greatly enhanced the 1 alpha,25(OH)2D3-induced fusion every time the macrophages were treated simultaneously with the two vitamins. The giant cells induced by the two vitamins were much larger than those induced by 1 alpha,25(OH)2D3 alone. The macrophages treated with 1 alpha,25(OH)2D3 started to fuse 36 h after the addition of the vitamin, whereas the macrophages pretreated with retinoic acid for 24 h began to fuse immediately after 1 alpha,25(OH)2D3 was added. 1 alpha,25(OH)2D3 and retinoic acid activated alveolar macrophages similarly, measured by the enhancement of glucose consumption and the induction of cytotoxicity against tumor cells, though 1 alpha,25(OH)2D3 was 100 times more potent than retinoic acid on a molar basis. Simultaneous treatment with physiological concentrations of 1 alpha,25(OH)2D3 (0.12 nM) and retinoic acid (10 nM) induced cytotoxicity additively. Morphological examinations revealed that the treated cells were enlarged and flattened with numerous filopodia. These results clearly indicate that both 1 alpha,25(OH)2D3 and retinoic acid similarly activate alveolar macrophages, and the activated state is prerequisite to the fusion of macrophages induced by 1 alpha,25(OH)2D3.
C Miyaura; A Segawa; H Nagasawa; E Abe; T Suda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research     Volume:  1     ISSN:  0884-0431     ISO Abbreviation:  J. Bone Miner. Res.     Publication Date:  1986 Aug 
Date Detail:
Created Date:  1988-11-14     Completed Date:  1988-11-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8610640     Medline TA:  J Bone Miner Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  359-68     Citation Subset:  IM    
Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
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MeSH Terms
Calcitriol / pharmacology*
Cell Fusion / drug effects
Cells, Cultured
Drug Synergism
Glucose / metabolism
Macrophage Activation / drug effects*
Macrophages / drug effects,  ultrastructure
Mice, Inbred Strains
Microscopy, Electron, Scanning
Pulmonary Alveoli / drug effects*
Tretinoin / pharmacology*
Reg. No./Substance:
302-79-4/Tretinoin; 32222-06-3/Calcitriol; 50-99-7/Glucose

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