| Effects of resveratrol on H(2)O(2)-induced apoptosis and expression of SIRTs in H9c2 cells. | |
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MedLine Citation:
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PMID: 19415680 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Resveratrol, a polyphenol found in fruits, has been demonstrated to activate Sir2. Though many studies have demonstrated that resveratrol can activate SIRT1, whether it has effect on other sirtuins (SIRT2-7) are unknown. The present study shows that exposure of H9c2 cells to 50 microM H(2)O(2) for 6 h caused a significant increase in apoptosis, as evaluated by TUNEL and flow cytometry (FCM), but pretreatment of resveratrol (20 microM) eliminated H(2)O(2)-induced apoptosis. Resveratrol also prevented H(2)O(2)-induced caspase-3 activation. Exposure of cells to resveratrol caused rapid activation of SIRT1,3,4,7. Sirtuin inhibitor, nicotinamide (20 mM) attenuated resveratrol's inhibitory effect on cell apoptosis and caspase-3 activity. These results suggest that resveratrol protects cardiomyocytes from H(2)O(2)-induced apoptosis by activating SIRT1,3,4,7. |
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Authors:
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Wei Yu; Yu-Cai Fu; Xiao-Hui Zhou; Chun-Juan Chen; Xin Wang; Rui-Bo Lin; Wei Wang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 107 ISSN: 1097-4644 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-06-30 Completed Date: 2009-10-26 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 741-7 Citation Subset: IM |
Copyright Information:
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2009 Wiley-Liss, Inc. |
Affiliation:
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Department of Cardiology, First Affiliated Hospital of Shantou University Medical College, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Caspase 3 / metabolism Cell Line Gene Expression Regulation / drug effects* Hydrogen Peroxide / pharmacology* Myocytes, Cardiac / cytology, drug effects* Rats Sirtuin 1 Sirtuins / genetics* Stilbenes / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Stilbenes; 501-36-0/resveratrol; 7722-84-1/Hydrogen Peroxide; EC 3.4.22.-/Caspase 3; EC 3.5.1.-/Sirt1 protein, rat; EC 3.5.1.-/Sirtuin 1; EC 3.5.1.-/Sirtuins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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