| Effects of respiratory syncytial virus infection and major basic protein derived from eosinophils in pulmonary alveolar epithelial cells (A549). | |
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MedLine Citation:
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PMID: 20977431 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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RSV (respiratory syncytial virus)-induced pneumonia and bronchiolitis may be associated with hyperresponsive conditions, including asthma. Eosinophilic proteins such as MBP (major basic protein) may also be associated with the pathophysiology of asthma. To elucidate the roles of RSV infection and MBP in the pathogenesis of pneumonia with hyperresponsiveness, we investigated the effects of RSV infection and MBP on A549 (alveolar epithelial) cells. CPE (cytopathic effects) in A549 cells were observed by microscopy. Apoptosis and cell death was evaluated by flow cytometric analysis and modified MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. We also measured 15 types of cytokines and chemokines in A549 cell supernatants. Although RSV alone did not affect the CPE of A549, high concentrations of MBP resulted in cell death within 24 h. Combinations of RSV and MBP synergistically induced cell death. In A549 cells infected with RSV alone, the release of GM-CSF (granulocyte-macrophage colony-stimulating factor) was significantly enhanced compared with control cells (no infection). In the cells treated with MBP alone, the production of IL (interleukin)-2, 4, 5, 7, 10, 12, 13, 17, IFN (interferon)-γ, GM-CSF, G-CSF (granulocyte colony-stimulating factor) and MIP (macrophage inflammatory protein)-1β was significantly increased compared with control cells. Notably, the levels of GM-CSF and IL-17 in RSV/MBP-treated cells were significantly higher than those treated with MBP alone. These results suggest that MBP synergistically enhanced the release of various cytokines/chemokines and the cell death of RSV-infected A549 cells, indicating that MBP may be closely associated with the pathophysiology of allergic reactions in bronchiolitis/pneumonia due to RSV. |
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Authors:
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Taisei Ishioka; Hirokazu Kimura; Hirohito Kita; Masatsugu Obuchi; Hiroo Hoshino; Masahiro Noda; Atsuyoshi Nishina; Kunihisa Kozawa; Masahiko Kato |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell biology international Volume: 35 ISSN: 1095-8355 ISO Abbreviation: Cell Biol. Int. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
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Languages: eng Pagination: 467-74 Citation Subset: IM |
Affiliation:
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Gunma Prefectural Institute of Public Health and Environmental Sciences, 378 Kamioki-machi, Maebashi-shi, Gunma, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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