| Effects of resistin expression on glucose metabolism and hepatic insulin resistance. | |
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MedLine Citation:
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PMID: 19184634 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to observe the effect of increased serum resistin on glucose metabolism, insulin sensitivity, and hepatic insulin resistance (IR), mice were intravenously injected with recombinant adenovirus carrying the resistin gene (Adv-resistin-EGFP). Changes in hepatic glucose metabolism were observed using the Periodic Acid-Schiff method. Hepatic AMP-activated protein kinase (AMPK) activation was assessed by Western blot analysis, and glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK) mRNA expression was determined using real-time RT-PCR. Although no effect on fasting blood glucose was detected, increased fasting insulin levels, decreased glucose tolerance and insulin sensitivity, and reduced hepatic glycogen levels and AMPK activation were seen in the Adv-resistin-EGFP mice. Finally, elevated G6Pase and PEPCK mRNA expression levels were detected upon overexpression of resistin. Resistin may inhibit hepatic AMPK activity, which results in elevated expression of gluconeogenic enzymes thereby affecting glucose metabolism and leading to decreased glycogen storage that contributes to the development of hepatic IR. |
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Authors:
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Fang-Ping Li; Juan He; Zhi-Zhen Li; Zhao-Fan Luo; Li Yan; Yan Li |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-01-31 |
Journal Detail:
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Title: Endocrine Volume: 35 ISSN: 1355-008X ISO Abbreviation: Endocrine Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-03-24 Completed Date: 2009-06-18 Revised Date: 2010-06-24 |
Medline Journal Info:
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Nlm Unique ID: 9434444 Medline TA: Endocrine Country: United States |
Other Details:
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Languages: eng Pagination: 243-51 Citation Subset: IM |
Affiliation:
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Department of Endocrinology and Metabolism Department, The Second Affiliated Hospital, Sun Yat-sen University, No. 107 Yanjiang West Road, Guangzhou 510120, China. Amiy36@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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AMP-Activated Protein Kinases
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metabolism Adenoviridae / genetics Animals Blood Glucose / analysis Blotting, Western Enzyme Activation Fasting Gene Expression* Genetic Vectors Glucose / metabolism* Glucose Intolerance Glucose-6-Phosphatase / genetics Glycogen / analysis Insulin / blood Insulin Resistance / physiology* Liver / chemistry, drug effects*, enzymology Male Mice Mice, Inbred C57BL Phosphoenolpyruvate Carboxykinase (GTP) / genetics RNA, Messenger / analysis Recombinant Proteins / genetics Resistin / genetics*, physiology Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/RNA, Messenger; 0/Recombinant Proteins; 0/Resistin; 11061-68-0/Insulin; 50-99-7/Glucose; 9005-79-2/Glycogen; EC 2.7.11.1/AMP-Activated Protein Kinases; EC 3.1.3.9/Glucose-6-Phosphatase; EC 4.1.1.32/Phosphoenolpyruvate Carboxykinase (GTP) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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