| Effects of reproductive stage and 11-ketotestosterone on LPL mRNA levels in the ovary of the shortfinned eel. | |
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MedLine Citation:
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PMID: 20713648 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To understand the dynamics of lipid uptake into the ovary and the potential role that lipoprotein lipase plays in this event, changes in LPL transcript abundance during oogenesis were measured in both wild-caught and pituitary homogenate-induced artificially maturing eels. Also, the effects of 11-ketotestosterone (11-KT) on LPL mRNA levels were investigated in vivo and in vitro. Normalized ovarian LPL transcript abundance increased as oogenesis advanced, and it rose particularly rapidly during midvitellogenesis, corresponding to pronounced increases in ovarian lipid deposits and LPL activity. Furthermore, LPL mRNA levels were dramatically increased following 11-KT treatment in vivo, findings that were reinforced as trends in ovarian tissue incubated in vitro. Ovarian LPL appears to be directly involved in the uptake of lipids into the eel ovary, an involvement that appears to be controlled, at least in part, by the androgen 11-KT. |
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Authors:
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Sean L Divers; H James McQuillan; Hajime Matsubara; Takashi Todo; P Mark Lokman |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-16 |
Journal Detail:
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Title: Journal of lipid research Volume: 51 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-13 Completed Date: 2011-01-19 Revised Date: 2011-11-01 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 3250-8 Citation Subset: IM |
Affiliation:
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Department of Zoology, University of Otago, Dunedin, New Zealand. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anguilla
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metabolism*,
physiology Animals Cell Size / drug effects Cloning, Molecular DNA, Complementary / genetics Female Lipid Metabolism / drug effects Lipoprotein Lipase / genetics*, metabolism Liver / drug effects, metabolism Oocytes / cytology, drug effects Ovary / drug effects*, enzymology, metabolism, physiology* RNA, Messenger / genetics, metabolism Reproduction / physiology* Testosterone / analogs & derivatives*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/RNA, Messenger; 53187-98-7/11-ketotestosterone; 58-22-0/Testosterone; EC 3.1.1.34/Lipoprotein Lipase |
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