Document Detail


Effects of rejuvenation and frozen storage on 42-day-old AS-3 RBCs.
MedLine Citation:
PMID:  14617310     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: A storage period of 42 days has been approved by the FDA for RBCs stored in NUTRICEL (AS-3). This study was undertaken to provide data to the FDA about the feasibility of salvaging AS-3 RBCs at the end of their storage period by rejuvenation and freezing, and to evaluate the effect of rejuvenation on indicators of RBC function. STUDY DESIGN AND METHODS: Healthy adults (n = 22) donated 450 mL of whole blood, and RBC components were prepared at two study sites. The components were stored at 1 to 6 degrees C for either 41 days (nonrejuvenated frozen controls, n = 6) or 42 days (rejuvenated frozen study group, n = 10; nonrejuvenated nonfrozen controls, n = 6). Rejuvenated study components and nonrejuvenated frozen controls were stored at -70 degrees C for longer than 2 weeks. Frozen units were then deglycerolized and kept for an additional 24 hours at 1 to 6 degrees C. RESULTS: 2,3-DPG, and ATP were reduced after 42-day storage to near 0 and 65 percent, respectively, of their original values. After rejuvenation and deglycerolization, the mean ATP level was 146 percent and the mean 2,3-DPG was 115 percent. The percent freeze-thaw-wash recovery was similar for rejuvenated and nonrejuvenated RBCs. Trace amounts of hypoxanthine and inosine were detected in rejuvenated units. The mean 24-hour survival (single- or double-label technique) of all components exceeded 75 percent. The t1/2 of study and control RBCs was similar. CONCLUSION: The ability of 42-day-old AS-3 RBCs to deliver oxygen after rejuvenation and freezing is not impaired. These data indicate that rejuvenated AS-3 RBCs can provide a safe and beneficial blood component immediately upon infusion.
Authors:
William B Lockwood; Russell W Hudgens; Irula O Szymanski; Richard A Teno; Alan D Gray
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transfusion     Volume:  43     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-11-17     Completed Date:  2003-12-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1527-32     Citation Subset:  IM    
Affiliation:
American Red Cross Blood Services, River Valley Region, Louisville, Kentucky, USA. wblock01@gwise.louisville.edu
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MeSH Terms
Descriptor/Qualifier:
2,3-Diphosphoglycerate / blood
Adenosine Triphosphate / blood
Adult
Cell Survival
Cryopreservation*
Erythrocytes* / metabolism,  physiology*
Feasibility Studies
Female
Humans
Hypoxanthine / blood
Inosine / blood
Male
Middle Aged
Rejuvenation
Time Factors
Chemical
Reg. No./Substance:
138-81-8/2,3-Diphosphoglycerate; 56-65-5/Adenosine Triphosphate; 58-63-9/Inosine; 68-94-0/Hypoxanthine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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