Document Detail

Effects of red light emitting diode on apoptosis of HeLa cells and suppression of implanted HeLa cells growth in mice.
MedLine Citation:
PMID:  19164885     Owner:  NLM     Status:  MEDLINE    
Low intensity irradiation of cells by laser was an effective method of biostimulation. Here, we have extended these actions to evaluate the apoptosis effects in red light emitting diode (RLED) exposure. Through morphological observation, flow cytometric analysis, intracellular calcium measurement and RT-PCR, we found that HeLa cells in 24 h RLED irradiation in in-vitro experiments would significantly affects the induction of cellular apoptosis, and morphological changes such as the loose arrangement of cells, the noticeable development of apoptotic bodies,and the accompaniment of arrested S phase and activated caspases-3,-6,-8. Moreover, intracellular calcium concentrations markedly increased 40.3 +/- 1.3% and 43.1 +/- 0.8% respectively, relative to an extracellular solution containing the Ca(2+) and Ca(2+) free unexposed group. In in-vivo tests, RLED irradiation decreased the growth of tumors on day 50 and attenuated the elevation of vascular endothelial growth factor (VEGF) expression in HeLa cell implanted BALB/c mice. Taken together, our results suggest that RLED could induce HeLa cell apoptosis and convey potential antitumor properties.
Li Zhang; Zhe Xiong; Zhengjia Li; Bei Yao; Donghua Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-22
Journal Detail:
Title:  Journal of radiation research     Volume:  50     ISSN:  0449-3060     ISO Abbreviation:  J. Radiat. Res.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-04-06     Completed Date:  2009-07-01     Revised Date:  2012-03-28    
Medline Journal Info:
Nlm Unique ID:  0376611     Medline TA:  J Radiat Res     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  109-17     Citation Subset:  IM    
Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union of Medical College, Tianjin, China.
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MeSH Terms
Calcium / metabolism
Caspases / biosynthesis
Cell Cycle / radiation effects
Flow Cytometry / methods
HeLa Cells
Mice, Inbred BALB C
Neoplasm Transplantation
Neoplasms, Experimental / radiotherapy*
Tetrazolium Salts / pharmacology
Thiazoles / pharmacology
Reg. No./Substance:
0/Tetrazolium Salts; 0/Thiazoles; 298-93-1/thiazolyl blue; 7440-70-2/Calcium; EC 3.4.22.-/Caspases

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