| Effects of red light emitting diode on apoptosis of HeLa cells and suppression of implanted HeLa cells growth in mice. | |
MedLine Citation:
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PMID: 19164885 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Low intensity irradiation of cells by laser was an effective method of biostimulation. Here, we have extended these actions to evaluate the apoptosis effects in red light emitting diode (RLED) exposure. Through morphological observation, flow cytometric analysis, intracellular calcium measurement and RT-PCR, we found that HeLa cells in 24 h RLED irradiation in in-vitro experiments would significantly affects the induction of cellular apoptosis, and morphological changes such as the loose arrangement of cells, the noticeable development of apoptotic bodies,and the accompaniment of arrested S phase and activated caspases-3,-6,-8. Moreover, intracellular calcium concentrations markedly increased 40.3 +/- 1.3% and 43.1 +/- 0.8% respectively, relative to an extracellular solution containing the Ca(2+) and Ca(2+) free unexposed group. In in-vivo tests, RLED irradiation decreased the growth of tumors on day 50 and attenuated the elevation of vascular endothelial growth factor (VEGF) expression in HeLa cell implanted BALB/c mice. Taken together, our results suggest that RLED could induce HeLa cell apoptosis and convey potential antitumor properties. |
Authors:
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Li Zhang; Zhe Xiong; Zhengjia Li; Bei Yao; Donghua Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-01-22 |
Journal Detail:
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Title: Journal of radiation research Volume: 50 ISSN: 0449-3060 ISO Abbreviation: J. Radiat. Res. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-04-06 Completed Date: 2009-07-01 Revised Date: 2012-03-28 |
Medline Journal Info:
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Nlm Unique ID: 0376611 Medline TA: J Radiat Res Country: Japan |
Other Details:
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Languages: eng Pagination: 109-17 Citation Subset: IM |
Affiliation:
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Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union of Medical College, Tianjin, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* Calcium / metabolism Caspases / biosynthesis Cell Cycle / radiation effects Flow Cytometry / methods HeLa Cells Humans Light* Mice Mice, Inbred BALB C Neoplasm Transplantation Neoplasms, Experimental / radiotherapy* Tetrazolium Salts / pharmacology Thiazoles / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Tetrazolium Salts; 0/Thiazoles; 298-93-1/thiazolyl blue; 7440-70-2/Calcium; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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