Document Detail


Effects of reconstituted collagen matrix on fates of mouse embryonic stem cells before and after induction for chondrogenic differentiation.
MedLine Citation:
PMID:  19331579     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Embryonic stem (ES) cells are pluripotent cells with great potential in regenerative medicine. However, controlling their differentiation toward homogeneous lineages is challenging. In this study, we aim to investigate the effects of reconstituted 3D collagen matrix on the fates of mouse ES (mES) cells before and after induction for chondrogenic differentiation. Specifically, mES cells were encapsulated and cultured in 3D collagen microspheres and exposed to induction signals at different time points. Growth characteristics and differentiation status of mES cells were then evaluated. Collagen microspheres provided a suitable microenvironment supporting mES cell growth and maintaining their undifferentiated status for certain period of time. At later time points, the proportion of undifferentiated mES cells gradually decreased, accompanied by increasing proportions of mesenchymal progenitor cells. This suggests the inductive role of collagen matrix in differentiating mES cells toward mesenchymal lineages. Moreover, a lower initial collagen monomer concentration facilitated the differentiation of mES cells into chondrogenic lineages, while induction at a later time point associated with a more advanced stage of chondrogenic differentiation. This indicates that both the initial collagen concentration and the time to induce differentiation significantly affected the fates of mES cells. This study contributes to future development of ES cell-based therapies.
Authors:
Chiu W Yeung; Kathryn Cheah; Danny Chan; Barbara P Chan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  15     ISSN:  1937-335X     ISO Abbreviation:  -     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-08     Completed Date:  2009-12-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3071-85     Citation Subset:  IM    
Affiliation:
Medical Engineering Program, Department of Mechanical Engineering, The University of Hong Kong, Hong Kong Special Administrative Region, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / drug effects,  physiology*
Cell Proliferation / drug effects
Cell Survival / drug effects
Cells, Cultured
Chondrogenesis
Collagen / metabolism*,  pharmacology
Embryonic Stem Cells / cytology*,  drug effects
Extracellular Matrix / metabolism*
Flow Cytometry
Immunohistochemistry
Mice
Microspheres
SOX9 Transcription Factor / metabolism
Tissue Engineering / methods
Chemical
Reg. No./Substance:
0/SOX9 Transcription Factor; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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