Document Detail

Effects of reactive oxygen species on prostacyclin production in perinatal rat lung cells.
MedLine Citation:
PMID:  2651389     Owner:  NLM     Status:  MEDLINE    
A differentiation-arrested primary cell culture model was used to examine the role of reactive oxygen species in the control of prostacyclin (PGI2) production in the perinatal rat lung. Coincubation of the lung cells with arachidonic acid (AA) and xanthine (X, 0.25 mM) plus xanthine oxidase (XO, 10 mU/ml) or with AA and glucose (25 mM) plus glucose oxidase (25 mU/ml) augmented the AA-induced PGI2 output. Superoxide dismutase (10 U/ml) did not alter the X + XO effect, whereas catalase (10 U/ml) eliminated both X + XO and glucose plus glucose oxidase effects. H2O2 (1-200 microM) showed a dose-related biphasic augmentation with peak stimulation at 20 microM. Catalase again blocked this effect, but dimethylthiourea, a hydroxyl radical scavenger, did not. A 20-min pretreatment of the cells with X + XO, glucose plus glucose oxidase, or H2O2, however, diminished the capacity of the cells to convert exogenous AA to PGI2. This pretreatment effect was also blocked by catalase. The responses were similar in lung cells obtained from day 20 rat fetuses (term = 22 days) and 1-day-old newborn rats. Lactate dehydrogenase release was not detected during treatment periods but increased significantly after exposure to reactive oxygen species.(ABSTRACT TRUNCATED AT 250 WORDS)
D S Lee; E A McCallum; D M Olson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  66     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1989 Mar 
Date Detail:
Created Date:  1989-06-02     Completed Date:  1989-06-02     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1321-7     Citation Subset:  IM    
Department of Paediatrics, University of Western Ontario, London, Canada.
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MeSH Terms
6-Ketoprostaglandin F1 alpha / biosynthesis
Animals, Newborn
Arachidonic Acids / metabolism
Catalase / metabolism
Cells, Cultured
Epoprostenol / biosynthesis*
Free Radicals
Hydrogen Peroxide / pharmacology
Lung / drug effects,  embryology,  metabolism*
Oxygen / metabolism*
Superoxide Dismutase / metabolism
Xanthine Oxidase / metabolism
Xanthines / pharmacology
Reg. No./Substance:
0/Arachidonic Acids; 0/Free Radicals; 0/Xanthines; 35121-78-9/Epoprostenol; 58962-34-8/6-Ketoprostaglandin F1 alpha; 69-89-6/Xanthine; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen; EC; EC Dismutase; EC Oxidase

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