Document Detail

Effects of reactive oxygen species on cell proliferation and death in HeLa cells and its MDR1-overexpressing derivative cell line.
MedLine Citation:
PMID:  12576695     Owner:  NLM     Status:  MEDLINE    
In this paper, the effects of H2O2, a typical reactive oxygen species (ROS), on cell proliferation or death were examined using the human cervical carcinoma cell line HeLa and its MDR1-overexpressing subline, Hvr100-6, which was established by stepwise exposure to vinblastine. It was confirmed that the growth of HeLa cells was enhanced by H2O2 at relatively low concentrations in a concentration-dependent manner, and the growth enhancement was suppressed by antioxidants. Doxorubicin and daunorubicin also enhanced the growth of HeLa cells at concentrations lower than IC50 values, and the antioxidants suppressed this effect, being consistent with the fact that both anticancer drugs generate ROS. The growth enhancement by H2O2 or doxorubicin and daunorubicin was not observed in Hvr100-6 cells. In addition, it was suggested that antioxidants had no effect on MDR1 mRNA expression in HeLa and Hvr100-6 cells, and thereby hardly reverse multidrug resistance in tumor cells.
Yu-Wen Liu; Toshiyuki Sakaeda; Kohji Takara; Tsutomu Nakamura; Nobuko Ohmoto; Chiho Komoto; Hironao Kobayashi; Tatsurou Yagami; Noboru Okamura; Katsuhiko Okumura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  26     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-02-10     Completed Date:  2003-08-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  278-81     Citation Subset:  IM    
Department of Hospital Pharmacy, School of Medicine, Kobe University, Kobe, Japan.
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MeSH Terms
Cell Death / drug effects,  physiology
Cell Division / drug effects,  physiology
Dose-Response Relationship, Drug
Gene Expression Regulation / drug effects*,  physiology
Growth Inhibitors / pharmacology
Hela Cells
P-Glycoprotein / biosynthesis*
Reactive Oxygen Species / pharmacology*
Reg. No./Substance:
0/Growth Inhibitors; 0/P-Glycoprotein; 0/Reactive Oxygen Species

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