Document Detail


Effects of pyridoxalated hemoglobin polyoxyethylene conjugate and stroma-free hemoglobin on renal vascular responsiveness to vasoactive substances in isolated perfused rat kidney.
MedLine Citation:
PMID:  8060252     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of pyridoxalated hemoglobin polyoxyethylene conjugate (PHP) and stroma-free hemoglobin (SFH) on vascular responsiveness to various vasoactive substances were examined in isolated perfused rat kidneys. The kidneys isolated from rats were perfused with 6% PHP, 6% SFH, and 6% hydroxyethylstarch (HES) solution at a constant flow rate. Vascular responsiveness to acetylcholine (ACh), nitroglycerin (NG), norepinephrine (NE), and angiotensin-II (ANG-II) was examined by measuring the perfusion pressure (PP). Effects of inhibition of endothelium-derived relaxing factor (EDRF) by NG-monomethyl L-arginine (L-NMMA) on NE-induced and ANG-II-induced renal vascular responses were examined. ACh and NG induced a dose-dependent decrease in perfusion pressure (PP) in all groups. NE and ANG-II induced an increase in PP in all groups, but NE-induced and ANG-II-induced responses in the PHP-perfused and SFH-perfused groups were significantly larger than those in the HES-perfused group. L-NMMA did not alter vascular responsiveness to NE and ANG-II. These results indicate that PHP and SFH do not inhibit EDRF induced by ACh, but hemoglobin moiety per se does augment the vascular responsiveness to NE and ANG-II in the isolated perfused rat kidney.
Authors:
K Nishi; S Ueda; D Sato; K Nishi
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Artificial organs     Volume:  18     ISSN:  0160-564X     ISO Abbreviation:  Artif Organs     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-09-15     Completed Date:  1994-09-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7802778     Medline TA:  Artif Organs     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  429-38     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Kumamoto University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / pharmacology
Angiotensin II / pharmacology
Animals
Arginine / analogs & derivatives,  pharmacology
Blood Substitutes / pharmacology
Hemoglobins / pharmacology*
Male
Nitric Oxide / antagonists & inhibitors,  pharmacology
Nitroglycerin / pharmacology
Norepinephrine / pharmacology
Perfusion
Polyethylene Glycols / pharmacology*
Rats
Rats, Wistar
Renal Circulation / drug effects*
Vasoconstrictor Agents / pharmacology*
Vasodilator Agents / pharmacology*
omega-N-Methylarginine
Chemical
Reg. No./Substance:
0/Blood Substitutes; 0/Hemoglobins; 0/Polyethylene Glycols; 0/Vasoconstrictor Agents; 0/Vasodilator Agents; 0/hemoglobin, stroma free; 0/pyridoxalated-hemoglobin-polyethylene glycol conjugate; 10102-43-9/Nitric Oxide; 11128-99-7/Angiotensin II; 17035-90-4/omega-N-Methylarginine; 51-41-2/Norepinephrine; 51-84-3/Acetylcholine; 55-63-0/Nitroglycerin; 74-79-3/Arginine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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