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EFFECTS OF PUROMYCIN ON THE NUCLEOPROTEINS OF THE HELA CELL.
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MedLine Citation:
PMID:  14206418     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of several concentrations of puromycin on the nucleoproteins of HeLa cells grown in monolayers were studied by cytochemical and biochemical techniques. The earliest change at all concentrations of puromycin was a decrease in a granular form of ribonucleoprotein (RNP) that is demonstrable in the normal HeLa cell by the toluidine blue-molybdate (TBM) stain. The other types of RNP revealed by the TBM method were unaltered although the cell volume decreased markedly. Treatment with high concentrations of the antimetabolite resulted in pre-prophase inhibition of mitotic division and led to production of inclusions containing RNP in the cytoplasm; lower concentrations resulted in metaphase arrest. Biochemical analyses confirmed the cytochemical observations and indicated that synthesis of RNA and protein was inhibited to the same extent.
Authors:
G P STUDZINSKI; R LOVE
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  22     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1964 Sep 
Date Detail:
Created Date:  1965-01-01     Completed Date:  1996-12-01     Revised Date:  2011-09-28    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  493-503     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleus*
Cytoplasm*
Histocytochemistry*
Metabolism*
Nucleoproteins*
Pharmacology*
Puromycin*
RNA*
Research*
Tissue Culture Techniques*
Chemical
Reg. No./Substance:
0/Nucleoproteins; 53-79-2/Puromycin; 63231-63-0/RNA
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Copyright © 1964 by The Rockefeller Institute Press
Received Day: 7 Month: 11 Year: 1963
Print publication date: Day: 1 Month: 9 Year: 1964
Volume: 22 Issue: 3
First Page: 493 Last Page: 503
ID: 2106475
PubMed Id: 14206418

EFFECTS OF PUROMYCIN ON THE NUCLEOPROTEINS OF THE HELA CELL
G. P. Studzinski
R. Love
From the Department of Pathology, Jefferson Medical College, Philadelphia


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