Document Detail

Effects of purified Salvia miltiorrhiza extract on cardiac vascular smooth muscle hypoxic cells.
MedLine Citation:
PMID:  17652908     Owner:  NLM     Status:  MEDLINE    
Recently, we have reported that purified Salvia miltiorrhiza extract (PSME) could prevent myocardial infarction in vivo and myocardial ischemia/reperfusion injury in isolated rat hearts (ex vivo). The aim of this project is to determine whether PSME exerts any cardioprotective effects in vitro. The vascular smooth muscle cell line was used and the effects of the drugs were determined after inducing hypoxia. Gene expression levels of the pro-apoptotic genes Asp53, Bax, and Fas were significantly down-regulated by 0.78-, 0.82-, and 0.87-fold, respectively, and Bcl-2 was up-regulated by 0.82-fold in the PSME-treated groups as compared to the hypoxic group (P<0.05). Significant reduction in immunoreactivity of the protein products of these genes as well as least nuclear green fluorescence observed in TUNEL staining indicate the therapeutic potential of this drug. Furthermore, cardiac antioxidant enzymes assay confirmed this deduction as PSME had slight preserving effects on superoxide dismutase and catalase (0.25 +/- 0.01 vs 0.488 +/- 0.02 units/mg protein and 0.026 +/- 0.012 vs 0.076 +/- 0.01 mumol per min per mg protein, respectively; each P<0.05). No significant results were obtained with glutathione S-transferase and GSH peroxidase antioxidant tests. Our results demonstrated that PSME exerts antioxidant effects in vitro, indicating the therapeutic potential of this drug.
Begum Husna; Todd On; Yi Zhun Zhu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmacological sciences     Volume:  104     ISSN:  1347-8613     ISO Abbreviation:  J. Pharmacol. Sci.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-26     Completed Date:  2007-09-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101167001     Medline TA:  J Pharmacol Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  202-11     Citation Subset:  IM    
Department of Pharmacology, Faculty of Medicine, National University of Singapore, 119260, Singapore.
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MeSH Terms
Antioxidants / metabolism
Cell Hypoxia / physiology*
Cell Survival / drug effects
Cells, Cultured
Electrophoresis, Agar Gel
Gene Expression / drug effects
In Situ Nick-End Labeling
L-Lactate Dehydrogenase / metabolism
Muscle, Smooth, Vascular / cytology*,  drug effects
Myocardial Ischemia / pathology,  prevention & control
Myocardium / cytology*
Plant Extracts / pharmacology
RNA / biosynthesis,  isolation & purification
Reverse Transcriptase Polymerase Chain Reaction
Salvia miltiorrhiza / chemistry*
Trypan Blue
Reg. No./Substance:
0/Antioxidants; 0/Plant Extracts; 63231-63-0/RNA; 72-57-1/Trypan Blue; EC Dehydrogenase

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