| Effects of purified Salvia miltiorrhiza extract on cardiac vascular smooth muscle hypoxic cells. | |
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MedLine Citation:
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PMID: 17652908 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recently, we have reported that purified Salvia miltiorrhiza extract (PSME) could prevent myocardial infarction in vivo and myocardial ischemia/reperfusion injury in isolated rat hearts (ex vivo). The aim of this project is to determine whether PSME exerts any cardioprotective effects in vitro. The vascular smooth muscle cell line was used and the effects of the drugs were determined after inducing hypoxia. Gene expression levels of the pro-apoptotic genes Asp53, Bax, and Fas were significantly down-regulated by 0.78-, 0.82-, and 0.87-fold, respectively, and Bcl-2 was up-regulated by 0.82-fold in the PSME-treated groups as compared to the hypoxic group (P<0.05). Significant reduction in immunoreactivity of the protein products of these genes as well as least nuclear green fluorescence observed in TUNEL staining indicate the therapeutic potential of this drug. Furthermore, cardiac antioxidant enzymes assay confirmed this deduction as PSME had slight preserving effects on superoxide dismutase and catalase (0.25 +/- 0.01 vs 0.488 +/- 0.02 units/mg protein and 0.026 +/- 0.012 vs 0.076 +/- 0.01 mumol per min per mg protein, respectively; each P<0.05). No significant results were obtained with glutathione S-transferase and GSH peroxidase antioxidant tests. Our results demonstrated that PSME exerts antioxidant effects in vitro, indicating the therapeutic potential of this drug. |
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Authors:
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Begum Husna; Todd On; Yi Zhun Zhu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of pharmacological sciences Volume: 104 ISSN: 1347-8613 ISO Abbreviation: J. Pharmacol. Sci. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-07-26 Completed Date: 2007-09-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101167001 Medline TA: J Pharmacol Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 202-11 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Faculty of Medicine, National University of Singapore, 119260, Singapore. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / metabolism Cell Hypoxia / physiology* Cell Survival / drug effects Cells, Cultured Electrophoresis, Agar Gel Gene Expression / drug effects Immunohistochemistry In Situ Nick-End Labeling L-Lactate Dehydrogenase / metabolism Muscle, Smooth, Vascular / cytology*, drug effects Myocardial Ischemia / pathology, prevention & control Myocardium / cytology* Plant Extracts / pharmacology RNA / biosynthesis, isolation & purification Rats Reverse Transcriptase Polymerase Chain Reaction Salvia miltiorrhiza / chemistry* Trypan Blue |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Plant Extracts; 63231-63-0/RNA; 72-57-1/Trypan Blue; EC 1.1.1.27/L-Lactate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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