Document Detail


Effects of purified Salvia miltiorrhiza extract on cardiac vascular smooth muscle hypoxic cells.
MedLine Citation:
PMID:  17652908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recently, we have reported that purified Salvia miltiorrhiza extract (PSME) could prevent myocardial infarction in vivo and myocardial ischemia/reperfusion injury in isolated rat hearts (ex vivo). The aim of this project is to determine whether PSME exerts any cardioprotective effects in vitro. The vascular smooth muscle cell line was used and the effects of the drugs were determined after inducing hypoxia. Gene expression levels of the pro-apoptotic genes Asp53, Bax, and Fas were significantly down-regulated by 0.78-, 0.82-, and 0.87-fold, respectively, and Bcl-2 was up-regulated by 0.82-fold in the PSME-treated groups as compared to the hypoxic group (P<0.05). Significant reduction in immunoreactivity of the protein products of these genes as well as least nuclear green fluorescence observed in TUNEL staining indicate the therapeutic potential of this drug. Furthermore, cardiac antioxidant enzymes assay confirmed this deduction as PSME had slight preserving effects on superoxide dismutase and catalase (0.25 +/- 0.01 vs 0.488 +/- 0.02 units/mg protein and 0.026 +/- 0.012 vs 0.076 +/- 0.01 mumol per min per mg protein, respectively; each P<0.05). No significant results were obtained with glutathione S-transferase and GSH peroxidase antioxidant tests. Our results demonstrated that PSME exerts antioxidant effects in vitro, indicating the therapeutic potential of this drug.
Authors:
Begum Husna; Todd On; Yi Zhun Zhu
Related Documents :
24793158 - Patient-specific finite element modeling of the cardiokinetix parachute(®) device: eff...
10443918 - Lipid peroxidation and alterations to oxidative metabolism in mitochondria isolated fro...
17630348 - Cytoprotective effects of n,n,n-trimethylsphingosine during ischemia- reperfusion injur...
19394208 - Protective effects of purified safflower extract on myocardial ischemia in vivo and in ...
6641248 - Left ventricular function and extent of coronary artery disease in the absence of antec...
20063618 - Collaborative working empowers staff to cut the number of cardiac arrests.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmacological sciences     Volume:  104     ISSN:  1347-8613     ISO Abbreviation:  J. Pharmacol. Sci.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-26     Completed Date:  2007-09-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101167001     Medline TA:  J Pharmacol Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  202-11     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Faculty of Medicine, National University of Singapore, 119260, Singapore.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / metabolism
Cell Hypoxia / physiology*
Cell Survival / drug effects
Cells, Cultured
Electrophoresis, Agar Gel
Gene Expression / drug effects
Immunohistochemistry
In Situ Nick-End Labeling
L-Lactate Dehydrogenase / metabolism
Muscle, Smooth, Vascular / cytology*,  drug effects
Myocardial Ischemia / pathology,  prevention & control
Myocardium / cytology*
Plant Extracts / pharmacology
RNA / biosynthesis,  isolation & purification
Rats
Reverse Transcriptase Polymerase Chain Reaction
Salvia miltiorrhiza / chemistry*
Trypan Blue
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Plant Extracts; 63231-63-0/RNA; 72-57-1/Trypan Blue; EC 1.1.1.27/L-Lactate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Predictors of target lesion revascularization and documented stent thrombosis beyond 30 days after s...
Next Document:  p38 mitogen-activated protein kinase mediates sidestream cigarette smoke-induced endothelial permeab...