Document Detail

Effects of pure FSH and LH preparations on the number and function of Leydig cells in immature hypophysectomized rats.
MedLine Citation:
PMID:  2493065     Owner:  NLM     Status:  MEDLINE    
The effects of pure FSH and/or LH preparations on the number of Leydig cells and their function in immature hypophysectomized rats have been investigated. As a result of hypophysectomy at the age of 17-18 days, the number of recognizable Leydig cells per testis decreased, as did the steroidogenic capacity in vivo and in vitro. Treatment with 64 micrograms FSH on both 22 and 23 days of age, did not affect the number of recognizable Leydig cells. In contrast, two injections of LH (10 micrograms) caused a sixfold increase in the number of Leydig cells, but had a negative effect on spermatogenesis. These stimulatory and inhibitory effects of LH diminished when FSH was added. Treatment with FSH for 7 days caused a twofold increase in the number of Leydig cells when compared with hypophysectomized controls. 3 beta-Hydroxysteroid dehydrogenase (3 beta-HSD) and esterase activity in Leydig cells also increased under the influence of FSH. The pregnenolone production per Leydig cell in the presence of 5-cholesten-3 beta,22(R)-diol (22R-hydroxycholesterol) as substrate showed a sevenfold increase. Plasma testosterone levels 2 h after injection of human chorionic gonadotrophin in intact rats and hypophysectomized FSH-treated rats were the same. Following LH treatment for 7 days, the number of Leydig cells proved to be 11 times higher, and 3 beta-HSD and esterase activity were not different from intact controls. The testicular pregnenolone production was four- to fivefold higher when compared with untreated hypophysectomized rats. However, pregnenolone production per Leydig cell in LH-treated rats was only slightly different from the hypophysectomized controls.(ABSTRACT TRUNCATED AT 250 WORDS)
K J Teerds; J Closset; F F Rommerts; D G de Rooij; D M Stocco; B Colenbrander; C J Wensing; G Hennen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of endocrinology     Volume:  120     ISSN:  0022-0795     ISO Abbreviation:  J. Endocrinol.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-04-05     Completed Date:  1989-04-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  97-106     Citation Subset:  IM    
Department of Cell Biology, Medical School, State University of Utrecht, The Netherlands.
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MeSH Terms
Cell Count
Follicle Stimulating Hormone / pharmacology*
Leydig Cells / drug effects*
Luteinizing Hormone / pharmacology*
Pregnenolone / metabolism
Rats, Inbred Strains
Spermatogenesis / drug effects
Testosterone / metabolism
Reg. No./Substance:
145-13-1/Pregnenolone; 58-22-0/Testosterone; 9002-67-9/Luteinizing Hormone; 9002-68-0/Follicle Stimulating Hormone

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